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Phosphoproteomic analysis of the Dahl sensitive rat

ABSTRACT: We analyzed the glomerular proteome in Dahl salt sensitive rats after 21 days of induction of hypertension, after 7 days and in control conditions.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Kidney

SUBMITTER: Markus Rinschen

LAB HEAD: Markus Rinschen

PROVIDER: PXD007937 | Pride | 2019-11-20

REPOSITORIES: Pride

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			Action	DRS
	20170929_Q2_JK_ColID_415_projID_781_Benzing_Sample_1.index	Other
	20170929_Q2_JK_ColID_415_projID_781_Benzing_Sample_1.raw	Raw
	20170929_Q2_JK_ColID_415_projID_781_Benzing_Sample_10.index	Other
	20170929_Q2_JK_ColID_415_projID_781_Benzing_Sample_10.raw	Raw
	20170929_Q2_JK_ColID_415_projID_781_Benzing_Sample_11.index	Other

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Publications

Metabolic rewiring of the hypertensive kidney.

Rinschen Markus M MM Palygin Oleg O Guijas Carlos C Palermo Amelia A Palacio-Escat Nicolas N Domingo-Almenara Xavier X Montenegro-Burke Rafael R Saez-Rodriguez Julio J Staruschenko Alexander A Siuzdak Gary G

Science signaling 20191210 611

Hypertension is a persistent epidemic across the developed world that is closely associated with kidney disease. Here, we applied a metabolomic, phosphoproteomic, and proteomic strategy to analyze the effect of hypertensive insults on kidneys. Our data revealed the metabolic aspects of hypertension-induced glomerular sclerosis, including lipid breakdown at early disease stages and activation of anaplerotic pathways to regenerate energy equivalents to counter stress. For example, branched-chain a ...[more]

PMID: 31822592

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Proteomic analysis of rat glomeruli from the salt sensitive Dahl rat

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Transcription profiling of rat Dahl salt-sensitive strain development of proteinuria

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Comparison of renal transcript levels in Dahl Salt-sensitive (S) rat with S.R congenic rat containing a BP QTL

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Project description:Diabetic nephropathy (DN) is a leading cause of ESRD worldwide, but its molecular pathogenesis is not well-defined and there are no specific treatments. In humans, there is a strong genetic component determining susceptibility to DN. However, specific genes controlling DN susceptibility in humans have not been identified. Here we describe a new mouse model, combining type 1 diabetes with activation of the renin angiotensin system (RAS), which develops robust kidney disease with features resembling human DN: heavy albuminuria, hypertension and glomerulosclerosis. Additionally, there is a powerful effect of genetic background regulating susceptibility to nephropathy. The 129 strain is susceptible to kidney disease, whereas the C57BL/6 strain is resistant. To examine the molecular basis of this differential susceptibility, we analyzed the glomerular transcriptome of young mice with albuminuria but without detectable alterations in glomerular structure. We find dramatic difference in regulation of immune and inflammatory pathways with up-regulation of pro-inflammatory pathways in the susceptible (129) strain and coordinate down-regulation in the resistant (C57BL/6) strain, compared to their respective baselines. Many of these pathways were also up-regulated in a rat model and in humans with DN. Our studies suggest that genes controlling inflammatory responses, triggered by hyperglycemia and hypertension, may be critical early determinants of susceptibility to DN. The analysis was carried out on 2 strains of mice (129/SvEv and C57BL/6), each involving 2 genotypes (wild-type and RenTg/Ins2Akita mutations). Four replicates were used for each strain-genotype (with the exception of 129/SvEv wild-type mice, which had 3 replicates).

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Gene expression analysis of rat glomerular parietal epithelial cells

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2014-08-01 | E-GEOD-54085 | biostudies-arrayexpress

Expression data from TNF-stimulated mouse glomeruli

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Comparison of Glomerular Transcriptome Profiles of Adult-Onset Steroid Sensitive Focal Segmental Glomerulosclerosis and Minimal Change Disease

Project description:To search for biomarkers to differentiate Adult-Onset Steroid Sensitive focal segmental glomerulosclerosis (FSGS) and minimal change disease (MCD). Compared the profiles of glomerular transcriptomes between patients with FSGS and patients with MCD using microarray analysis. This dataset is part of the TransQST collection.

2017-01-04 | E-GEOD-69814 | biostudies-arrayexpress

Cardiac transcriptome profiles of S.LEW congenic strain compared with the hypertensive Dahl S rat

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