Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Proteomic analysis of rat glomeruli from the salt sensitive Dahl rat

ABSTRACT: We analyzed the glomerular proteome of hypertensive rats developing FSGS.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Kidney

SUBMITTER: Markus Rinschen

LAB HEAD: Markus Rinschen

PROVIDER: PXD007940 | Pride | 2019-11-20

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	20170920_Q2_JK_ColID_415_projID_770_Benzing_Sample_1.index	Other
	20170920_Q2_JK_ColID_415_projID_770_Benzing_Sample_1.raw	Raw
	20170920_Q2_JK_ColID_415_projID_770_Benzing_Sample_2.index	Other
	20170920_Q2_JK_ColID_415_projID_770_Benzing_Sample_2.raw	Raw
	20170920_Q2_JK_ColID_415_projID_770_Benzing_Sample_3.index	Other

Items per page:

1 - 5 of 32

Publications

Metabolic rewiring of the hypertensive kidney.

Rinschen Markus M MM Palygin Oleg O Guijas Carlos C Palermo Amelia A Palacio-Escat Nicolas N Domingo-Almenara Xavier X Montenegro-Burke Rafael R Saez-Rodriguez Julio J Staruschenko Alexander A Siuzdak Gary G

Science signaling 20191210 611

Hypertension is a persistent epidemic across the developed world that is closely associated with kidney disease. Here, we applied a metabolomic, phosphoproteomic, and proteomic strategy to analyze the effect of hypertensive insults on kidneys. Our data revealed the metabolic aspects of hypertension-induced glomerular sclerosis, including lipid breakdown at early disease stages and activation of anaplerotic pathways to regenerate energy equivalents to counter stress. For example, branched-chain a ...[more]

PMID: 31822592

Similar Datasets

Transcription profiling of rat Dahl salt-sensitive strain development of proteinuria

Project description:To get more insight in cause and consequences of proteinuria, we studied glomerular gene expression patterns before and after the onset of increased urinary albumin excretion in a proteinuric rat strain. Spontaneously proteinuric Dahl salt-sensitive rats (Dahl SS) were compared to non-proteinuric, spontaneously hypertensive rats (SHR). In Dahl SS, UAE significantly increased starting from week 5 of age. Glomerular RNA profiles of 4- and 6 week-old rats were studied by Affymetrix microarrays. Experiment Overall Design: RNA was extracted from isolated glomeruli of 4- and 6 week-old rats from both strains. At each time point, two rats were used for each strain.

2009-01-14 | E-GEOD-13810 | biostudies-arrayexpress

Phosphoproteomic analysis of the Dahl sensitive rat

Project description:We analyzed the glomerular proteome in Dahl salt sensitive rats after 21 days of induction of hypertension, after 7 days and in control conditions.

2019-11-20 | PXD007937 | Pride

Comparison of Glomerular Transcriptome Profiles of Adult-Onset Steroid Sensitive Focal Segmental Glomerulosclerosis and Minimal Change Disease

Project description:To search for biomarkers to differentiate Adult-Onset Steroid Sensitive focal segmental glomerulosclerosis (FSGS) and minimal change disease (MCD). Compared the profiles of glomerular transcriptomes between patients with FSGS and patients with MCD using microarray analysis. This dataset is part of the TransQST collection.

2017-01-04 | E-GEOD-69814 | biostudies-arrayexpress

Transcription profiling of rat glomeruli: conventionally-isolated and laser capture microdissected glomeruli

Project description:We compared the gene expression analysis of 2 different glomerular isolation techniques (laser capture microdissection with 2 rounds of RNA amplification and unamplified glomerular RNA after iron perfusion with glomerular sieving) and obtained different results depending on the glomerular isolation technique that was used Experiment Overall Design: Paired kidneys from Lewis-Hsd rat kidneys were collected. The glomeruli of one kidney was obtained by LCM with 2 rounds of RNA amplification while the unamplified glomerular RNA of the contralateral kidney was obtained after perfusion with iron oxide and isolated by magnetic purification and a glomerular sieving technique. The RNA was analyzed using the Affymetrix Rat 230A microarrays.

2008-06-17 | E-GEOD-8998 | biostudies-arrayexpress

Targeted proteomic analysis of single glomeruli

Project description:WE analyzed the proteome of single mouse glomeruli using DDA and PRM.

2018-06-28 | PXD006903 | Pride

Microarray analysis of the development of proteinuria in the Dahl salt-sensitive rat

2009-01-01 | GSE13810 | GEO

Expression data from TNF-stimulated mouse glomeruli

Project description:The specific contribution of the two TNF-receptors Tnfr1 and Tnfr2 to TNF-induced inflammation in the glomerulus is unknown. In mice, TNF exposure induces glomerular expression of inflammatory mediators like adhesion molecules and chemokines in vivo, and glomerular accumulation of leukocytes. To examine Tnfr-specific inflammatory responses in intrinsic glomerular cells but not infiltrating leukocytes we performed microarray gene expression profiling on intact glomeruli isolated from wild-type and Tnfr-deficient mice following TNF exposure in vitro. Glomeruli were isolated from male C57BL/6J wild-type and Tnfr-deficient mice applying a magnetic bead-based isolation technique. Isolated intact glomeruli were stimulated with TNF for 12 hours in vitro for subsequent RNA extraction and hybridization on Affymetrix microarrays.

2013-09-11 | E-GEOD-43928 | biostudies-arrayexpress

Comparative phosphoproteomic analysis of mammalian renal glomeruli

Project description:Glomerular biology is dependent on tightly controlled signal transduction networks that control phosphorylation of signaling proteins such as cytoskeletal regulators or slit diaphragm proteins of kidney podocytes. Recent, in-depth analysis of the mouse glomerular phosphoproteome by tandem mass spectrometry confidently identified thousands of phosphorylation sites providing a unique opportunity to study signaling in vivo. However, the multitude of phosphorylated residues demands for additional systems biology approaches to separate physiologically important sites from non-functional phosphorylated residues. Cross-species comparison of phosphorylation events is a powerful mean to functionally prioritize and identify physiologically meaningful phosphorylation sites. Here, we present the result of additional phosphoproteomic analyses of the glomerular phosphoproteome in cow and rat to allow cross-species comparisons. We discovered several phosphorylation sites with potentially high biological relevance, e.g. tyrosine phosphorylation of the cytoskeletal regulator synaptopodin and the slit diaphragm protein neph-1 (Kirrel). Moreover, cross-species comparisons revealed conserved phosphorylation of the slit diaphragm protein nephrin on an acidic cluster at the intracellular terminus and conserved podocin phosphorylation on the very carboxyl terminus of the protein. Given the pivotal role of podocin for glomerular biology we studied a highly conserved podocin phosphorylation site in greater detail and show that phosphorylation regulates affinity of the interaction with nephrin and CD2AP. Taken together, these results suggest that species comparisons of phosphoproteomic data may reveal regulatory principles in glomerular biology.

2015-01-16 | PXD001005 | Pride

Targeted proteomics analysis of single glomeruli in Doxorubicin-induced FSGS

Project description:We analyzed the proteome of single glomeruli from mouse kidney with Doxorubicin-induced FSGS. We analyzed the proteomes by PRM.

2018-06-28 | PXD007144 | Pride

Mouse kidney glomeruli. Control vs. podocin(R231Q/A268V)

Project description:Similar to human patients, mice with the compound heterozygous mutation podocin R231Q/A268V, develop a late onset FSGS. Here, we did a proteomics anaylsis of kidney glomeruli before the onset of disease (mice at 3 weeks of age). Analysis of the data reveals that there are no developmental changes in the proteome of compund heterozygous mice.

2020-05-15 | PXD018326 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data