Proteomics

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Identification of H3 Lysine 4 Monomethylation Associated Proteins at Mammalian Enhancers


ABSTRACT: Enhancers act to regulate cell type specific gene expression by facilitating the transcription of target genes. In mammalian cells active or primed enhancers are commonly marked by monomethylation of Histone H3 at lysine 4 (H3K4me1) in a cell-type specific manner. Whether and how this histone modification regulates enhancer-dependent transcription programs in mammals has been unclear. In the present study, we conducted SILAC Mass-spec experiments with mono-nucleosomes and identified multiple H3K4me1 associated proteins, including proteins involved in chromatin remodeling. We demonstrate that H3K4me1 augments the association of the chromatin remodeling complex BAF to enhancers in vivo. Furthermore we show that in vitro, H3K4me1 nucleosomes are more efficiently remodeled by the BAF complex. Crystal structures of a BAF component BAF45c further reveal that monomethylation, but not trimethylation, is accommodated in this protein’s H3K4 binding site. Our results suggest that H3K4me1 plays an active role at enhancers by facilitating the binding of the BAF complex and possibly other chromatin regulators.

OTHER RELATED OMICS DATASETS IN: PRJNA317750

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Claudio Ponte de Albuquerque  

LAB HEAD: Bing Ren

PROVIDER: PXD007942 | Pride | 2018-10-23

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
14_11062012_HILIC1.RAW Raw
14_11062012_HILIC1.mzXML Mzxml
14_11062012_HILIC1.pep.xml Pepxml
14_11062012_HILIC10.RAW Raw
14_11062012_HILIC10.mzXML Mzxml
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Publications


Enhancers act to regulate cell-type-specific gene expression by facilitating the transcription of target genes. In mammalian cells, active or primed enhancers are commonly marked by monomethylation of histone H3 at lysine 4 (H3K4me1) in a cell-type-specific manner. Whether and how this histone modification regulates enhancer-dependent transcription programs in mammals is unclear. In this study, we conducted SILAC mass spectrometry experiments with mononucleosomes and identified multiple H3K4me1-  ...[more]

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