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Human Glioblastoma Cell Line Secretome LC-MS/MS

ABSTRACT: To investigate the effect of glioblastoma secretome in activating tau pathology, Glioblastoma cell line secretome were prefractionated with gel filtration chromatography. Secretome analyzed by LC-MS/MS and identified targets using PSM count quantitation.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

DISEASE(S): Glioblastoma

SUBMITTER: Shinyeong Ju

LAB HEAD: Cheolju Lee

PROVIDER: PXD007969 | Pride | 2018-07-16

REPOSITORIES: pride

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			Action	DRS
	140416_HS683_Tryp_HpH_F01.RAW	Raw
	140416_HS683_Tryp_HpH_F02.RAW	Raw
	140416_HS683_Tryp_HpH_F03.RAW	Raw
	140416_HS683_Tryp_HpH_F04.RAW	Raw
	140416_HS683_Tryp_HpH_F05.RAW	Raw

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Publications

Glioblastoma-secreted soluble CD44 activates tau pathology in the brain.

Lim Sungsu S Kim Dohee D Ju Shinyeong S Shin Seulgi S Cho Il-Joo IJ Park Sung-Hye SH Grailhe Regis R Lee Cheolju C Kim Yun Kyung YK

Experimental & molecular medicine 20180406 4

During aggressive tumor growth and migration, glioblastoma cells secrete diverse molecules and adhesion proteins to the extracellular matrix. Yet, the biochemical effects of the glioblastoma secretome in the brain remain largely unknown. Here we show that soluble CD44 secreted from glioblastoma cells induces neuronal degeneration through the activation of tau pathology in the brain. Glioblastoma-xenograft tissues showed a number of degenerating neurons bearing highly phosphorylated tau. Through ...[more]

PMID: 29622771

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Abernathy2016 - glioblastoma treatment

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