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Echis carinatus carinatus (India) venom proteomics

ABSTRACT: In order to study the proteome of Echis carinatus carinatus venom of Indian origin, crude venom was fractionated on a Shodex KW-803 gel filtration column coupled to Dionex Ultimate 3000 UHPLC system. The resulting peaks were pooled and subjected to in-solution trypsin digestion and subsequent tandem mass spectrometry analysis. The raw data were then analysed using PEAKS 8.5 software to finally decipher the complex venom proteome.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Echis Carinatus

TISSUE(S): Saliva

SUBMITTER: Aparup Patra

LAB HEAD: Dr. Ashis Kumar Mukherjee

PROVIDER: PXD007980 | Pride | 2017-12-15

REPOSITORIES: Pride

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			Action	DRS
	Ashis-1.RAW	Raw
	Ashis-10.RAW	Raw
	Ashis-2.RAW	Raw
	Ashis-3.RAW	Raw
	Ashis-4.RAW	Raw

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Publications

Proteomics and antivenomics of Echis carinatus carinatus venom: Correlation with pharmacological properties and pathophysiology of envenomation.

Patra Aparup A Kalita Bhargab B Chanda Abhishek A Mukherjee Ashis K AK

Scientific reports 20171207 1

The proteome composition of Echis carinatus carinatus venom (ECV) from India was studied for the first time by tandem mass spectrometry analysis. A total of 90, 47, and 22 distinct enzymatic and non-enzymatic proteins belonging to 15, 10, and 6 snake venom protein families were identified in ECV by searching the ESI-LC-MS/MS data against non-redundant protein databases of Viperidae (taxid 8689), Echis (taxid 8699) and Echis carinatus (taxid 40353), respectively. However, analysis of MS/MS data a ...[more]

PMID: 29215036

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