Dataset Information

First dataset - bacterial proteome of carbapanemase-producing Escherichia coli and other Enterobacteriaceae under antibiotic pressure

ABSTRACT: Antibiotic resistance associated with the expression of the clinically significant carbapenemases, IMP, KPC, and NDM and OXA-48 in Enterobacteriaceae is emerging as a worldwide calamity to health care. In Australia, IMP-producing Enterobacteriaceae is the most prevalent carbapenemase-producing Enterobacteriaceae (CPE). Genomic characteristics of such carbapenemase-producing Enterobacteriaceae (CPE) are well described, but the corresponding proteome is poorly characterised. We have thus developed a method to analyse dynamic changes in the proteome of CPE under antibiotic pressure. Specifically, we have investigated the effect of meropenem at sub-lethal concentrations to develop a better understanding of how antibiotic pressure leads to resistance. Escherichia coli, producing either NDM, IMP or KPC type carbapenemase were included in this study, and their proteomes were analysed in growth conditions with or without meropenem.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Escherichia Coli

DISEASE(S): Recurrent Urinary Tract Infections,Sepsis

SUBMITTER: Hanna Sidjabat

LAB HEAD: Hanna Sidjabat

PROVIDER: PXD008019 | Pride | 2018-07-11

REPOSITORIES: pride

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Dataset's files

Source:

			Action	DRS
	20140715WinterMNDCON234ANODATEE1BT25_15.group	Other
	20170623CONTROLAWSATH.wiff.scan	Wiff
	20170623CONTROLBWSATH.wiff.scan	Wiff
	20170623IMPEc1ASWATH.wiff.scan	Wiff
	20170623IMPEc1BSWATH.wiff.scan	Wiff

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Publications

The use of SWATH to analyse the dynamic changes of bacterial proteome of carbapanemase-producing Escherichia coli under antibiotic pressure.

Sidjabat Hanna E HE Gien Jolene J Kvaskoff David D Ashman Keith K Vaswani Kanchan K Reed Sarah S McGeary Ross P RP Paterson David L DL Bordin Amanda A Schenk Gerhard G

Scientific reports 20180301 1

Antibiotic resistance associated with the clinically significant carbapenemases KPC, NDM and OXA-48 in Enterobacteriaceae is emerging as worldwide. In Australia, IMP-producing Enterobacteriaceae are the most prevalent carbapenemase-producing Enterobacteriaceae (CPE). Genomic characteristics of such CPE are well described, but the corresponding proteome is poorly characterised. We have thus developed a method to analyse dynamic changes in the proteome of CPE under antibiotic pressure. Specificall ...[more]

PMID: 29497067

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