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SILAC proteome of MTase deficient cells

ABSTRACT: SILAC proteome of MTase deficient HAP-1 cells and corresponding WT.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Magnus Jakobsson

LAB HEAD: Jesper Velgaard Olsen

PROVIDER: PXD008131 | Pride | 2018-07-04

REPOSITORIES: pride

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			Action	DRS
	20160517_QE5_nLC14_MJ_HAP1_SILAC_Rep01_Frac01.raw	Raw
	20160517_QE5_nLC14_MJ_HAP1_SILAC_Rep01_Frac02.raw	Raw
	20160517_QE5_nLC14_MJ_HAP1_SILAC_Rep01_Frac03.raw	Raw
	20160517_QE5_nLC14_MJ_HAP1_SILAC_Rep01_Frac04.raw	Raw
	20160517_QE5_nLC14_MJ_HAP1_SILAC_Rep01_Frac05.raw	Raw

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Publications

The dual methyltransferase METTL13 targets N terminus and Lys55 of eEF1A and modulates codon-specific translation rates.

Jakobsson Magnus E ME Małecki Jędrzej M JM Halabelian Levon L Nilges Benedikt S BS Pinto Rita R Kudithipudi Srikanth S Munk Stephanie S Davydova Erna E Zuhairi Fawzi R FR Arrowsmith Cheryl H CH Jeltsch Albert A Leidel Sebastian A SA Olsen Jesper V JV Falnes Pål Ø PØ

Nature communications 20180824 1

Eukaryotic elongation factor 1 alpha (eEF1A) delivers aminoacyl-tRNA to the ribosome and thereby plays a key role in protein synthesis. Human eEF1A is subject to extensive post-translational methylation, but several of the responsible enzymes remain unknown. Using a wide range of experimental approaches, we here show that human methyltransferase (MTase)-like protein 13 (METTL13) contains two distinct MTase domains targeting the N terminus and Lys55 of eEF1A, respectively. Our biochemical and str ...[more]

PMID: 30143613

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