Ontology highlight
ABSTRACT:
INSTRUMENT(S): autoflex
ORGANISM(S): Cyprinus Carpio (common Carp)
TISSUE(S): Semen
SUBMITTER: Mariola Dietrich
LAB HEAD: Mariola Dietrich
PROVIDER: PXD008187 | Pride | 2018-03-23
REPOSITORIES: Pride
Action | DRS | |||
---|---|---|---|---|
CRYOSUPERNATANT_01.dat | Other | |||
CRYOSUPERNATANT_01.mgf | Mgf | |||
CRYOSUPERNATANT_01.zip | Other | |||
CRYOSUPERNATANT_02.dat | Other | |||
CRYOSUPERNATANT_02.mgf | Mgf |
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PloS one 20180322 3
Our recent studies suggested that the freezability of carp semen is related to seminal plasma protein profiles. Here, we aimed to compare the spermatozoa proteomes of good (GF) and poor (PF) freezability semen of carp. To achieve this, we used two-dimensional difference in gel electrophoresis followed by MALDI-TOF/TOF mass spectrometry. The semen was classified as GF or PF based on sperm motility after freeze/thawing. We identified proteins enriched in spermatozoa of GF (22 proteins) and PF (18 ...[more]