Proteomics

Dataset Information

0

Comparison of Protein Expressions of Drug-Metabolizing Enzymes between Human Liver and the Hepatic Cell Lines HepG2, Hep3B and Huh7 using SWATH and MRM-HR Proteomics

ABSTRACT: Human hepatic cell lines have been widely used as an in vitro model for the study of drug metabolism and liver toxicity. However, the validity of this model is still a subject of debate because the expressions of various proteins including drug-metabolizing enzymes (DMEs) in the cell lines can differ significantly from that of human livers. In the present study, we first conducted an untargeted proteomics of the microsomes of the cell lines HepG2, Hep3B, and Huh7 in comparison with human livers using a SWATH method. Furthermore, a targeted proteomic approach, named high-resolution multiple reaction monitoring (MRM-HR), was utilized to compare the expressions of pre-selected DMEs between human livers and the cell lines.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hepatocyte, Liver, Cell Culture

SUBMITTER: Jian Shi  

LAB HEAD: Hao-Jie Zhu

PROVIDER: PXD008190 | Pride | 2018-04-18

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
HLM1_IDA_8ug_20170406_inj1.wiff Wiff
HLM1_IDA_8ug_20170406_inj1.wiff.scan Wiff
HLM1_MRM-HR_5ug.wiff Wiff
HLM1_MRM-HR_5ug.wiff.scan Wiff
HLM1_SWATH_5ug.wiff Wiff
Items per page:
1 - 5 of 81
altmetric image

Publications

Comparison of protein expression between human livers and the hepatic cell lines HepG2, Hep3B, and Huh7 using SWATH and MRM-HR proteomics: Focusing on drug-metabolizing enzymes.

Shi Jian J   Wang Xinwen X   Lyu Lingyun L   Jiang Hui H   Zhu Hao-Jie HJ  

Drug metabolism and pharmacokinetics 20180310 2

Human hepatic cell lines are widely used as an in vitro model for the study of drug metabolism and liver toxicity. However, the validity of this model is still a subject of debate because the expressions of various proteins in the cell lines, including drug-metabolizing enzymes (DMEs), can differ significantly from those in human livers. In the present study, we first conducted an untargeted proteomics analysis of the microsomes of the cell lines HepG2, Hep3B, and Huh7, and compared them to huma  ...[more]

Similar Datasets

Proteomics A Novel QconCAT-Based Proteomics Method for Determining Allele-Specific Protein Expression (ASPE)
2018-08-28 | PXD008788 | Pride
Proteomics Inosine induces context-dependent recoding and translational stalling
2018-11-06 | PXD010329 | Pride
Genomics Gene expressions of human gastric cancer cell lines
| PRJNA638140 | ENA
Proteomics Proteomic analysis of Psmd9 overexpression in the liver
2019-11-01 | PXD007285 | Pride
Proteomics Proteomic analysis of Psmd9 knockdown in the liver
2019-03-14 | PXD010759 | Pride
Transcriptomics Expression data of hepatocytes isolated from chimeric mouse livers repopulated with human hepatocytes and from normal human livers
2012-11-27 | GSE33846 | GEO
Transcriptomics Transcriptional profiling of hepatocytes isolated from chronically HBV-infected human liver chimeric mice
2020-05-22 | GSE145835 | GEO
Proteomics,Multiomics HipSci: the iPSC proteomic compendium
2019-07-25 | PXD010557 | Pride
Genomics Mus musculus
| PRJNA273349 | ENA
Proteomics Hippocampus Proteomics analysis of 5×FAD::Alb-CreERT2;Ephx2−/− mice and control littermates (5×FAD::Ephx2loxp/loxp); Note: Hepatic Ephx2 deletion in 5×FAD::Alb-CreERT2;Ephx2−/− mice
2023-06-22 | PXD037361 | Pride