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Multi-omic biogeography of the gastrointestinal microbiota of a pre-weaned lamb

ABSTRACT: Here, we used reverse-phase liquid chromatography-coupled tandem mass spectrometry to study the pre-weaned lamb proteome and metaproteome in ten different gastrointestinal tracts: rumen, reticulum, omasum, abomasum, duodenum, jejunum, ileum, cecum, colon, and rectum.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Microbiota Ovis Aries

TISSUE(S): Reticulum, Jejunum, Duodenum, Omasum, Cecum, Rectum, Rumen, Colon, Ileum, Abomasum

SUBMITTER: Antonio Palomba

LAB HEAD: Sergio Uzzau

PROVIDER: PXD008192 | Pride | 2017-12-14

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	01_Rumen_luminal.raw	Raw
	01_Rumen_luminal_DB-Host.msf	Msf
	01_Rumen_luminal_DB1.msf	Msf
	01_Rumen_luminal_DB2.msf	Msf
	01_Rumen_mucosal.raw	Raw

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Publications

Multi-Omic Biogeography of the Gastrointestinal Microbiota of a Pre-Weaned Lamb.

Palomba Antonio A Tanca Alessandro A Fraumene Cristina C Abbondio Marcello M Fancello Francesco F Atzori Alberto Stanislao AS Uzzau Sergio S

Proteomes 20171218 4

The digestive functions of the pre-weaned lamb gastrointestinal tracts (GITs) have been the subject of much research in recent years, but the microbial and host functions underlying these complex processes remain largely unknown. Here, we undertook a proof-of-principle metaproteogenomic investigation on luminal and mucosal samples collected from 10 GITs of a 30-day-old pre-weaned lamb. We demonstrate that the analysis of the diverse ecological niches along the GITs can reveal microbiota composit ...[more]

PMID: 29258228

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