Dataset Information

Mosquito-Derived Anophelin Sulfoproteins are Potent Anti-Thrombotics

ABSTRACT: we first expressed AAa and AAg in a eukaryotic system to investigate whether the proteins could be sulfated in vivo. For this purpose we used insect cells as a model of the Anopheles mosquito. Specifically, codon-optimized sequences encoding AAa and AAg were designed as N-terminal fusions with the honeybee mellitin signal sequence in order to direct the recombinant proteins to the secretion pathway and were expressed in Trichoplusia ni insect cells. Following expression, the cell medium containing the secreted proteins was analyzed by nanoliquid chromatography coupled to tandem mass spectrometry (nanoLC-MS/MS).

INSTRUMENT(S): Orbitrap Fusion ETD

ORGANISM(S): Anopheles Albimanus Anopheles Gambiae (african Malaria Mosquito)

SUBMITTER: Benjamin Parker

LAB HEAD: Benjamin Parker

PROVIDER: PXD008240 | Pride | 2018-08-30

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20170609_Fusion_BP_RichPayne_Baclo_Anophelin_Digest.raw	Raw
	20170609_Fusion_BP_RichPayne_Baclo_Anophelin_Digest_2.raw	Raw
	20170609_Fusion_BP_RichPayne_Baclo_Anophelin_Intact.raw	Raw
	20170609_Fusion_BP_RichPayne_Baclo_Anophelin_Intact_2.raw	Raw
	20170814_Fusion_BP_ETD_RichPayne_AnophelinSynthetic_Y12_300fmol_1.raw	Raw

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Publications

Mosquito-Derived Anophelin Sulfoproteins Are Potent Antithrombotics.

Watson Emma E EE Liu Xuyu X Thompson Robert E RE Ripoll-Rozada Jorge J Wu Mike M Alwis Imala I Gori Alessandro A Loh Choy-Theng CT Parker Benjamin L BL Otting Gottfried G Jackson Shaun S Pereira Pedro José Barbosa PJB Payne Richard J RJ

ACS central science 20180328 4

The anophelins are small protein thrombin inhibitors that are produced in the salivary glands of the Anopheles mosquito to fulfill a vital role in blood feeding. A bioinformatic analysis of anophelin sequences revealed the presence of conserved tyrosine residues in an acidic environment that were predicted to be post-translationally sulfated in vivo. To test this prediction, insect cell expression of two anophelin proteins, from Anopheles albimanus and Anopheles gambiae</ ...[more]

PMID: 29721529

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