Dataset Information

Identification of novel LIMK2 phosphorylation substrates

ABSTRACT: Breast cancer is the most common and a lethal form of cancer diagnosed in women worldwide. Although, effective treatment options for primary tumors are available, the majority of breast cancer-related deaths is caused by invasion of cells from primary tumor results in metastatic progression. Here we used a phopshoproteomic analysis to identify new targets of pro-invasive LIM kinase 2 (LIMK2) in invasive breast cancer cell lines. We show that Serine Arginine protein kinase 1 (SRPK1) is phosphorylated and activated by LIMK2 and LIMK2-SRPK1 signaling axis mediate the invasive behavior of breast cancer cells.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

DISEASE(S): Breast Cancer

SUBMITTER: narendra wajapeyee

LAB HEAD: Narendra Wajapeyee

PROVIDER: PXD008246 | Pride | 2020-08-31

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	OTF16-4008_M14_SILAC_PhosphoElution_Ted_inj02.raw	Raw
	SILAC_LIMK2_PhosphoELution.xml	Xml

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Publications

LIMK2 promotes the metastatic progression of triple-negative breast cancer by activating SRPK1.

Malvi Parmanand P Janostiak Radoslav R Chava Suresh S Manrai Padmini P Yoon Esther E Singh Kamaljeet K Harigopal Malini M Gupta Romi R Wajapeyee Narendra N

Oncogenesis 20200828 8

Triple-negative breast cancer (TNBC) is a highly metastatic breast cancer subtype and due to the lack of hormone receptors and HER2 expression, TNBC has limited therapeutic options with chemotherapy being the primary choice for systemic therapy. LIM Domain Kinase 2 (LIMK2) is a serine/threonine kinase that plays an important role in the regulation of actin filament dynamics. Here, we show that LIM domain kinase 2 (LIMK2) is overexpressed in TNBC, and short-hairpin RNA (shRNA)-mediated LIMK2 knoc ...[more]

PMID: 32859889

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