Proteomics

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Tyrosine-1 of RNA polymerase II CTD controls global termination of gene transcription in mammals

ABSTRACT: The aim of the project was to analyse the functional role of Tyrosine-1 of mammalian RNA polymerase II CTD in the regulation of transcription-coupled processes. We studied mammalian CTD mutants to analyze the function of tyrosine1 residues. Mutation of 3/4 of the tyrosine residues in a mutant YFFF resulted in a massive read-through transcription phenotype. Read-through was observed for 3’ processed and polyadenylated RNAs. The YFFF mutant shows a loss of interaction with the Mediator and Integrator complexes in MS analysis.

OTHER RELATED OMICS DATASETS IN: PRJNA369388

INSTRUMENT(S): LTQ Orbitrap, Q Exactive

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Ignasi Forne  

LAB HEAD: Prof. Dr. Dirk Eick

PROVIDER: PXD008270 | Pride | 2018-01-09

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
MS_rWTandYFFFmutants.xlsx Xlsx
Proteingroups_IF_20160919_NS.xlsx Xlsx
Ref547_NS_WTrec_150212.raw Raw
Ref547_NS_YF3_150212.raw Raw
Ref620_NS_WTrec_bead_160122.raw Raw
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Publications

The carboxy-terminal domain (CTD) of RNA polymerase (Pol) II is composed of a repetition of YSPTSPS heptads and functions as a loading platform for protein complexes that regulate transcription, splicing, and maturation of RNAs. Here, we studied mammalian CTD mutants to analyze the function of tyrosine1 residues in the transcription cycle. Mutation of 3/4 of the tyrosine residues (YFFF mutant) resulted in a massive read-through transcription phenotype in the antisense direction of promoters as w  ...[more]

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