Dataset Information

Ran asters proteomics - Proteomic profiling of microtubule self-organization in M-phase

ABSTRACT: The main proteomics events driving the self-organization of MTs in M-phase cytoplasms are determined by obtaining the full proteomes of the RanGTP induced MT structures at different time points in Xenopus egg extracts.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Xenopus Laevis (african Clawed Frog)

TISSUE(S): Egg Cell

SUBMITTER: guadalupe espadas

LAB HEAD: Eduard Sabidó

PROVIDER: PXD008306 | Pride | 2018-07-11

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	1311105_S_TCIV_01_01_15min_H.raw	Raw
	1311105_S_TCIV_02_02_15min_MH.raw	Raw
	1311105_S_TCIV_03_01_15min_ML.raw	Raw
	1311105_S_TCIV_04_01_15min_L.raw	Raw
	1311105_S_TCIV_05_01_20min_H.raw	Raw

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Publications

Proteomic Profiling of Microtubule Self-organization in M-phase.

Rosas-Salvans Miquel M Cavazza Tommaso T Espadas Guadalupe G Sabido Eduard E Vernos Isabelle I

Molecular & cellular proteomics : MCP 20180703 10

Microtubules (MTs) and associated proteins can self-organize into complex structures such as the bipolar spindle, a process in which RanGTP plays a major role. Addition of RanGTP to M-phase <i>Xenopus</i> egg extracts promotes the nucleation and self-organization of MTs into asters and bipolar-like structures in the absence of centrosomes or chromosomes. We show here that the complex proteome of these RanGTP-induced MT assemblies is similar to that of mitotic spindles. Using proteomic profiling ...[more]

PMID: 29970457

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