Proteomics

Dataset Information

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Rat, urine, LC-MS/MS - Two predominant MUPs, OBP3 and MUP13, are male pheromones in rats


ABSTRACT: Two MUPs of rat urine, odorant-binding protein 3 (OBP3) and MUP13, were identified by LC-MS/MS,and found to be male-biased and androgen-dependent and then confirmed to be male pheromones in rats.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Urine

DISEASE(S): Disease Free

SUBMITTER: guo xiao  

LAB HEAD: Jian-Xu Zhang

PROVIDER: PXD008341 | Pride | 2018-02-26

REPOSITORIES: Pride

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Publications

Two predominant MUPs, OBP3 and MUP13, are male pheromones in rats.

Guo Xiao X   Guo Huifen H   Zhao Lei L   Zhang Yao-Hua YH   Zhang Jian-Xu JX  

Frontiers in zoology 20180223


<h4>Background</h4>In rats, urine-borne male pheromones comprise organic volatile compounds and major urinary proteins (MUPs). A number of volatile pheromones have been reported, but no MUP pheromones have been identified in rat urine.<h4>Results</h4>We used sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), isoelectric focusing electrophoresis (IEF), nano-liquid chromatography-tandem mass spectrometry (nLC-MS/MS) after in gel digestion of the proteins and quantitative real-t  ...[more]

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