Quantitative proteomics of the 2016 WHO Neisseria gonorrhoeae reference strains identifies vaccine candidates and antimicrobial resistance signatures.

ABSTRACT: Neisseria gonorrhoeae is the causative agent of gonorrhea, a leading sexually transmitted disease with severe complications on reproductive health. The U.S. Centers for Disease Control and Prevention has categorized the public health threat induced by N. gonorrhoeae as “urgent”, due to the ease of transmission and the fast emergence of multi-drug resistant strains. The need for development of vaccines and understanding the underlying factors leading to antibiotic resistance is of utmost importance. The proteomic profiles of the 14 WHO N. gonorrhoeae reference strains have been compared to the WHO F reference strain using a mass spectrometry with tandem mass tags (TMT) labeling to analyze the cell envelope and the cytoplasmic fractions extracted from each strain. Identifying novel vaccine candidates and proteomic signatures for antimicrobial resistance will further our understanding of N. gonorrhoeae proteotypes, in relationship to their respective genotypes and phenotypes, and provide deep insights that will impact the development of preventive and therapeutic tools to combat gonorrhea.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Neisseria Gonorrhoeae Fa6140 Neisseria Gonorrhoeae

TISSUE(S): Prokaryotic Cell

DISEASE(S): Gonorrhea

SUBMITTER: Fadi El-Rami  

LAB HEAD: Aleksandra Sikora

PROVIDER: PXD008412 | Pride | 2018-12-19

REPOSITORIES: Pride