Dataset Information

Comparative phosphoproteomic analysis reveals signaling networks regulating monopolar and bipolar cytokinesis

ABSTRACT: Successful completion of cytokinesis requires the coordinated activities of diverse cellular components including membranes, cytoskeletal elements and chromosomes. Biochemical analysis of cytokinesis is challenging due to its dynamic and rapid nature that necessitates the establishment of robust methodologies. Our comparative proteomic approach suggests that monopolar cytokinesis is a good surrogate for cytokinesis and it is a well-suited system for global biochemical analysis in mammalian cells. We established a phosphoproteomic signature of cytokinesis. More than 10,000 phosphorylation sites were systematically screened and monitored; around 800 of these phosphosites were up-regulated during cytokinesis.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hela Cell

SUBMITTER: Erdem Sanal

LAB HEAD: Nurhan Özlü

PROVIDER: PXD008451 | Pride | 2018-02-07

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	AP_20140214_HELA_SCX_F30.raw	Raw
	AP_20140214_HELA_SCX_F31.raw	Raw
	AP_20140214_HeLa_SCX_F25.raw	Raw
	AP_20140214_HeLa_SCX_F26.raw	Raw
	AP_20140214_HeLa_SCX_F27.raw	Raw

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Comparative phosphoproteomic analysis reveals signaling networks regulating monopolar and bipolar cytokinesis.

Karayel Özge Ö Şanal Erdem E Giese Sven H SH Üretmen Kagıalı Zeynep Cansu ZC Polat Ayşe Nur AN Hu Chi-Kuo CK Renard Bernhard Y BY Tuncbag Nurcan N Özlü Nurhan N

Scientific reports 20180202 1

The successful completion of cytokinesis requires the coordinated activities of diverse cellular components including membranes, cytoskeletal elements and chromosomes that together form partly redundant pathways, depending on the cell type. The biochemical analysis of this process is challenging due to its dynamic and rapid nature. Here, we systematically compared monopolar and bipolar cytokinesis and demonstrated that monopolar cytokinesis is a good surrogate for cytokinesis and it is a well-su ...[more]

PMID: 29396449

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