Proteomics

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Peptidomic analysis of pancreatic neurodendocrine tumours (Glucagonoma)

ABSTRACT: A peptidomic analysis of plasma from patients with well characterised pancreatic neuroendocrine tumours (PNET) was performed. A nano LC-MS analysis identified a number of peptides from the glucagon gene, which were idenfiried in a previous case study using multiple immunoassays. Peptides to other proteins involved in peptide secretion were also identified. Plasma from a second glucagonoma patient was analysed using a high flow approach, and this identified similar peptides to the nano analysis. In order to demonstrate the potential clinical application of LC-MS to characterising neuroendocrine tumours, a large cohort of plasma samples were analysed to demonstrate the ability of this approach to identify glucagonoma patients, and differentiate from a single insulinoma patient

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma

DISEASE(S): Pancreatic Endocrine Carcinoma

SUBMITTER: Richard Kay  

LAB HEAD: Professor Fiona Gribble

PROVIDER: PXD008465 | Pride | 2018-06-06

REPOSITORIES: pride

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Publications

Peptidomic analysis of endogenous plasma peptides from patients with pancreatic neuroendocrine tumours.

Kay Richard G RG   Challis Benjamin G BG   Casey Ruth T RT   Roberts Geoffrey P GP   Meek Claire L CL   Reimann Frank F   Gribble Fiona M FM  

Rapid communications in mass spectrometry : RCM 20180801 16

<h4>Rationale</h4>Diagnosis of pancreatic neuroendocrine tumours requires the study of patient plasma with multiple immunoassays, using multiple aliquots of plasma. The application of mass spectrometry based techniques could reduce the cost and amount of plasma required for diagnosis.<h4>Methods</h4>Plasma samples from two patients with pancreatic neuroendocrine tumours were extracted using an established acetonitrile-based plasma peptide enrichment strategy. The circulating peptidome was charac  ...[more]

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