Dataset Information

Palaeoproteomic Profiling of Conservation Layers on a 14th Century Italian Wall Painting

ABSTRACT: Ahead of display, a non-original layer was observed on the surface of a fragment of a wall painting by Ambrogio Lorenzetti (active 1319, died 1348/9). FTIR analysis suggested proteinaceous content. Mass spectrometry was used to better characterise this layer and revealed two protein components: sheep and cow glue and chicken and mallard egg white. Analysis of posttranslational modifications detected several photo-oxidation products, which suggest that the egg experienced prolonged exposure to UV-light and was likely applied long before the addition of the glue. Additionally, glycation products detected may indicate naturally occurring glycoprotein degradation or reaction with a carbohydrate material such as starch, identified by ATR-FTIR in a cross-section of a sample taken from the painting. Palaeoproteomics is shown to provide detailed characterisation of organic layers associated with mural paintings and therefore aids reconstruction of the conservation history of these objects.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Bos Taurus (bovine) Gallus Gallus (chicken) Ovis Aries Anas Platyrhynchos

TISSUE(S): Egg, Skin, Bone, Tendon

SUBMITTER: Meaghan Mackie

LAB HEAD: Enrico Cappellini

PROVIDER: PXD008486 | Pride | 2018-04-05

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20170721_QE7_nLC11_MEM_COLLAB_GeoG_1228.raw	Raw
	20170721_QE7_nLC11_MEM_COLLAB_GeoG_1229.raw	Raw
	20170721_QE7_nLC11_MEM_COLLAB_GeoG_1230.raw	Raw
	MaxQuantSearch1.zip	Other
	MaxQuantSearch2.zip	Other

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Publications

Palaeoproteomic Profiling of Conservation Layers on a 14th Century Italian Wall Painting.

Mackie Meaghan M Rüther Patrick P Samodova Diana D Di Gianvincenzo Fabiana F Granzotto Clara C Lyon David D Peggie David A DA Howard Helen H Harrison Lynne L Jensen Lars Juhl LJ Olsen Jesper V JV Cappellini Enrico E

Angewandte Chemie (International ed. in English) 20180426 25

Ahead of display, a non-original layer was observed on the surface of a fragment of a wall painting by Ambrogio Lorenzetti (active 1319, died 1348/9). FTIR analysis suggested proteinaceous content. Mass spectrometry was used to better characterise this layer and revealed two protein components: sheep and cow glue and chicken and duck egg white. Analysis of post-translational modifications detected several photo-oxidation products, which suggest that the egg experienced prolonged exposure to UV l ...[more]

PMID: 29603563

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