Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive
ORGANISM(S): Mus Musculus (mouse)
TISSUE(S): Brain, Neuron
SUBMITTER: Maria Faelth Savitski
LAB HEAD: Marcus Bantscheff
PROVIDER: PXD008514 | Pride | 2018-06-08
REPOSITORIES: Pride
Items per page: 1 - 5 of 366 |
Nature communications 20180215 1
A better understanding of proteostasis in health and disease requires robust methods to determine protein half-lives. Here we improve the precision and accuracy of peptide ion intensity-based quantification, enabling more accurate protein turnover determination in non-dividing cells by dynamic SILAC-based proteomics. This approach allows exact determination of protein half-lives ranging from 10 to >1000 h. We identified 4000-6000 proteins in several non-dividing cell types, corresponding to 9699 ...[more]