Dataset Information

Proteomic characterization of Aspergillus fumigatus isolated from air and surfaces of the International Space Station

ABSTRACT: The on-going Microbial Observatory Experiments on the International Space Station (ISS) revealed the presence of various microorganisms that may be affected by the distinct environment of the ISS. The low-nutrient environment combined with enhanced irradiation and microgravity may trigger changes in the molecular suit of microorganisms leading to increased virulence and resistance of microbes. Proteomic characterization of two Aspergillus fumigatus strains, ISSFT-021 and IF1SW-F4, isolated from HEPA filter debris and cupola surface of the ISS, respectively, is presented, along with a comparison to experimentally established clinical isolates Af293 and CEA10. In-depth analysis highlights variations in the proteome of both ISS-isolated strains when compared to the clinical strains. Proteins up-regulated in ISS isolates were involved in oxidative stress response, and carbohydrate and secondary metabolism. This report provides insight into possible molecular adaptation of filamentous fungi to the unique ISS environment. Lastly, an attempt was made to elucidate plausible causes of the enhanced virulence of both ISS-isolated A. fumigatus strains.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Aspergillus Fumigatus Af293

TISSUE(S): Vegetative Cell (sensu Fungi)

SUBMITTER: Abby Chiang

LAB HEAD: Markus Kalkum

PROVIDER: PXD008517 | Pride | 2019-01-09

REPOSITORIES: pride

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Proteomic characterization of Aspergillus fumigatus isolated from air and surfaces of the International Space Station.

Blachowicz Adriana A Chiang Abby J AJ Romsdahl Jillian J Kalkum Markus M Wang Clay C C CCC Venkateswaran Kasthuri K

Fungal genetics and biology : FG & B 20190103

The on-going Microbial Observatory Experiments on the International Space Station (ISS) revealed the presence of various microorganisms that may be affected by the distinct environment of the ISS. The low-nutrient environment combined with enhanced irradiation and microgravity may trigger changes in the molecular suite of microorganisms leading to increased virulence and resistance of microbes. Proteomic characterization of two Aspergillus fumigatus strains, ISSFT-021 and IF1SW-F4, isolated from ...[more]

PMID: 30611835

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			Action	DRS
	JPLAFGM.msf	Msf
	JPLAFGM.pep.xml	Pepxml
	ac161014of006.raw	Raw
	ac161014of008.raw	Raw
	ac161014of010.raw	Raw