Proteomics

Dataset Information

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Defining targets of the bacterial ADP-ribosyltransferase EspJ

ABSTRACT: Tyrosine phosphorylation is key for signal transduction from exogeneous stimuli, including the defence against pathogenic microbes. Pathogens have acquired mechanisms to subvert protein phosphorylation as a means to control host immune responses and facilitate invasion and dissemination. The bacterial effector protein EspJ is injected into host cells by pathogenic strains of Escherichia coli, Salmonella and Citrobacter rodentium where it can prevent phagocytosis via the inhibitory amidation and ADP ribosylation of Src kinase E310. Here, we found that EspJ can ADP ribosylate members of the Src, Abl, Csk, Tec and Syk kinase families. ADP ribosylation of Csk inhibits its kinase activity, giving EspJ ultimate control over the Src/Csk signalling axis.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Tissue Culture, Epithelial Cell, Enterocyte, Macrophage

DISEASE(S): Disease Free

SUBMITTER: Dominic Pollard  

LAB HEAD: Gad Frankel

PROVIDER: PXD008533 | Pride | 2018-03-16

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
A549.zip Other
Caco2.zip Other
DP03_HeLa_txt.zip Other
DP_1A.raw Raw
DP_1B.raw Raw
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Publications

Broad-Spectrum Regulation of Nonreceptor Tyrosine Kinases by the Bacterial ADP-Ribosyltransferase EspJ.

Pollard Dominic J DJ   Berger Cedric N CN   So Ernest C EC   Yu Lu L   Hadavizadeh Kate K   Jennings Patricia P   Tate Edward W EW   Choudhary Jyoti S JS   Frankel Gad G  

mBio 20180410 2

Tyrosine phosphorylation is key for signal transduction from exogenous stimuli, including the defense against pathogens. Conversely, pathogens can subvert protein phosphorylation to control host immune responses and facilitate invasion and dissemination. The bacterial effectors EspJ and SeoC are injected into host cells through a type III secretion system by enteropathogenic and enterohemorrhagic <i>Escherichia coli</i> (EPEC and EHEC, respectively), <i>Citrobacter rodentium</i>, and <i>Salmonel  ...[more]

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