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Proteomic analysis of cucumber (Cucumis sativus) fruit peel in response to pre-storage cold acclimation

ABSTRACT: To distinguish PsCA-responsive proteins, total proteins extracted from the non-acclimated fruit, the fruit exposed to 3 d of cold acclimation, the control fruit stored at 5oC for 12 d and the fruit exposed to 3d of PsCA treatment followed by 9 d of cold storage were separated by 2-DE, respectively. More than 750 protein spots were detected using PDQuest Advanced 2-D Gel Analysis software (Version 8.0) following the 2-DE gel analysis. Of the 750 spots, 58 that displayed significant and reproducible changes in abundance were isolated, and 56 spots were successfully characterized using MS/MS coupled with database searching, which matched 51 proteins. 38 proteins have scores greater than threshold scores of 61(P < 0.05) and they are regarded as the significant differentially- accumulated proteins (SDAPs).

INSTRUMENT(S): ultraflex

ORGANISM(S): Cucumis Sativus

TISSUE(S): Skin

SUBMITTER: Shijiang Zhu

LAB HEAD: Shijiang Zhu

PROVIDER: PXD008551 | Pride | 2018-10-22

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	GELofControl12d.tif	Other
	GELofNA0d.tif	Other
	GELofPsCA12d.tif	Other
	GELofPsCA3d.tif	Other
	MS-IMAGE.zip	Other

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Publications

Proteomic Analysis of Differentially Accumulated Proteins in Cucumber (<i>Cucumis sativus</i>) Fruit Peel in Response to Pre-storage Cold Acclimation.

Wang Bin B Shen Fei F Zhu Shijiang S

Frontiers in plant science 20180118

Harvested fruits are still living organs and respond to environmental stimuli. Low temperature storage is effective in extending life of harvested fruit, but it may also cause chilling injury. Cold acclimation has been shown to induce chilling tolerance in plants, but what proteomic changes caused by cold acclimation are related to defense against chilling stress remains largely unclear. Here, 3 d of pre-storage cold acclimation (PsCA) at 10°C reduced chilling injury and secondary disease severi ...[more]

PMID: 29403505

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