Proteomics

Dataset Information

0

Identification of salivary biomarkers for oral cancer with quantitative proteomics and two-phase verifying approaches


ABSTRACT: Oral cavity cancer is one of the most common cancer worldwide and oral cavity squamous cell carcinoma (OSCC) accounts for up to 90 percent of oral cancers. In Taiwan, OSCC is the fifth most common malignancy of male and causes more than 250 deaths per year. The poor outcome of OSCC patients is principally ascribed to the fact that this disease is often advanced at the time of diagnosis, suggesting that early detection and prevention of OSCC are urgently needed to reduce the cancer burden. Analysis of cancer-related body fluids is one of promising approaches to identify cancer-related molecules and biomarker candidates. To identify salivary biomarkers of OSCC, salivary samples of OSCC patients, individuals with oral potentially malignant disorders (OPMD), and healthy volunteers were collected. With isobaric tags for relative and absolute quantitation (iTRAQ)-based mass spectrometry (MS) analysis, 1838 proteins were identified. The levels of 102 proteins are found to be elevated in the saliva samples of OSCC patients compared to that of OPMD and healthy groups. To improve the feasibility of biomarkers, we used a two-phase verification to measure these candidates. Among them, salivary levels of AHSG, CFH, FGA, and SERPINA1 were determined to be significantly higher in the OSCC patients than in the healthy or in the OPMD group with both of MRM-MS and ELISA. Furthermore, the levels of these proteins were highly related to tumor size, lymph-node metastasis, and cancer stages. The results of this study not only provides a powerful strategy for verification of biomarker candidates, but also suggested that AHSG, CFH, FGA, and SERPINA1 could act as salivary biomarkers for OSCC detection.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Saliva

SUBMITTER: Chih-Ching Wu  

LAB HEAD: Chih-Ching Wu

PROVIDER: PXD008655 | Pride | 2019-08-15

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
1_01.raw Raw
1_02.raw Raw
1_03.raw Raw
1_04.raw Raw
1_05.raw Raw
Items per page:
1 - 5 of 135
altmetric image

Publications

Identification of Salivary Biomarkers for Oral Cancer Detection with Untargeted and Targeted Quantitative Proteomics Approaches.

Chu Hao-Wei HW   Chang Kai-Ping KP   Hsu Chia-Wei CW   Chang Ian Yi-Feng IY   Liu Hao-Ping HP   Chen Yi-Ting YT   Wu Chih-Ching CC  

Molecular & cellular proteomics : MCP 20190628 9


Oral cavity squamous cell carcinoma (OSCC) is one of the most common cancers worldwide. In Taiwan, OSCC is the fifth leading cause of cancer-related mortality and leads to 2800 deaths per year. The poor outcome of OSCC patients is principally ascribed to the fact that this disease is often advanced at the time of diagnosis, suggesting that early detection of OSCC is urgently needed. Analysis of cancer-related body fluids is one promising approach to identify biomarker candidates of cancers. To i  ...[more]

Similar Datasets

2019-11-12 | PXD015722 | Pride
2024-08-23 | PXD047535 | Pride
2024-08-23 | PXD047673 | Pride
2015-04-01 | E-GEOD-38532 | biostudies-arrayexpress
2013-05-02 | E-GEOD-37991 | biostudies-arrayexpress
2010-06-21 | E-GEOD-18532 | biostudies-arrayexpress
2016-07-26 | E-GEOD-84805 | biostudies-arrayexpress
2011-08-31 | E-GEOD-25099 | biostudies-arrayexpress
2015-01-07 | E-GEOD-45238 | biostudies-arrayexpress
2022-04-04 | PXD025701 | Pride