Proteomics

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The fourth instar in the silk gland of silkworm LC-MSMS

ABSTRACT: In the present study, the silk gland proteomes of the fourth instar and fourth molt were analyzed using liquid chromatography−tandem mass spectrometry. In total, 2654 proteins were identified from the silk gland. High abundance of ribosomal proteins and RR-motif chitin-binding proteins were identified during day 2 of the fourth instar (IV-2) larval developmental stage, and the expression of cuticular proteins analogous to peritrophin (CPAP)-motif chitin-binding proteins was higher during the fourth molt (IV-M). In all, nine enzymes were found to be involved in the chitin regeneration pathway in the silk gland. Among them, two chitinase and two chitin deacetylases were identified as CPAP-motif proteins.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Bombyx Mori (silk Moth) Insecta

TISSUE(S): Excretory Gland

SUBMITTER: Xiaolu Zhang  

LAB HEAD: Qingyou Xia

PROVIDER: PXD008677 | Pride | 2018-06-04

REPOSITORIES: Pride

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Comparative Proteome Analysis Reveals that Cuticular Proteins Analogous to Peritrophin-Motif Proteins are Involved in the Regeneration of Chitin Layer in the Silk Gland of Bombyx mori at the Molting Stage.

Zhang Xiaolu X   Chang Huaipu H   Dong Zhaoming Z   Zhang Yan Y   Zhao Dongchao D   Ye Lin L   Xia Qingyou Q   Zhao Ping P  

Proteomics 20180830 19

The silk gland of silkworm produces silk proteins during larval development. Many studies have long focused on the silk gland of the fifth instar larvae, but few have investigated this gland at other larval stages. In the present study, the silk gland proteomes of the fourth instar and fourth molt are analyzed using liquid chromatography-tandem mass spectrometry. In total, 2654 proteins are identified from the silk gland. A high abundance of ribosomal proteins and RR-motif chitin-binding protein  ...[more]

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