Dataset Information

Shotgun proteomics of chemosensory organs of Varroa destructor mite

ABSTRACT: Aims of the project is the identification of soluble chemosensory proteins in the olfactory organs of the honeybee mite Varroa destructor. Different families of soluble proteins acting as carriers for odorants have been identified in hexapods, while very limited information is available for other terrestrial Arthropoda. In mites and ticks olfactory organs are located on the distal part of the forelegs and on the capitulum appendages. These two body tagmata and the second pair of legs as control, have been dissected out of phoretics and reproductives Varroa destructor females and protein extracts have been analysed through shotgun proteomics. Protein identification and relative quantification (Label-free quantification, LFQ) has been performed using MaxQuant (version 1.5.8.3). Among the proteins more abundant in appendages bearing chemosensory organs, considering as a model hexapoda soluble olfactory proteins, we have searched for small secreted proteins whose structure include a hydrophobic binding pocket.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Varroa Destructor

SUBMITTER: Francesca Romana Dani

LAB HEAD: Francesca Romana Dani

PROVIDER: PXD008679 | Pride | 2018-04-12

REPOSITORIES: Pride

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			Action	DRS
	Toni_20170225_GM_143_A10.raw	Raw
	Toni_20170225_GM_143_A11.raw	Raw
	Toni_20170225_GM_143_A12.raw	Raw
	Toni_20170225_GM_143_A13.raw	Raw
	Toni_20170225_GM_143_A14.raw	Raw

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Publications

Proteomic analysis of chemosensory organs in the honey bee parasite Varroa destructor: A comprehensive examination of the potential carriers for semiochemicals.

Iovinella Immacolata I McAfee Alison A Mastrobuoni Guido G Kempa Stefan S Foster Leonard J LJ Pelosi Paolo P Dani Francesca Romana FR

Journal of proteomics 20180410

We have performed a proteomic analysis on chemosensory organs of Varroa destructor, the honey bee mite, in order to identify putative soluble carriers for pheromones and other olfactory cues emitted by the host. In particular, we have analysed forelegs, mouthparts (palps, chelicera and hypostome) and the second pair of legs (as control tissue) in reproductive and phoretic stages of the Varroa life cycle. We identified 958 Varroa proteins, most of them common to the different organs and stages. S ...[more]

PMID: 29653265

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