Dataset Information

Temperature-induced changes in wheat leaf and flower phosphoproteome

ABSTRACT: Wheat (Triticum ssp.) is one of the most important human food sources as well as livestock feed, but is very sensitive to changes in temperature. Specifically, processes during leaf growth and photosynthesis and during flower and seed development are affected by high temperature. While this has been investigated to some extent on physiological, developmental and molecular levels, very little is known about early signalling associated with an increase in temperature. Here, we probed the impact of an increase in temperature for 1 hour on the leaf and flower phosphoproteome of wheat. In order to identify differentially phosphorylated peptides, we used two wheat proteome databases, and could show the superiority of the new IWGSC genome. Our analyses of the leaf phosphoproteome further revealed differential phosphorylation of heat shock proteins, Photosystem I subunits, and plasma membrane intrinsic proteins, in addition to a number of potentially novel players.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Triticum Aestivum (wheat)

TISSUE(S): Plant Cell, Flower, Leaf

SUBMITTER: Tingting Zhu

LAB HEAD: Ive De Smet

PROVIDER: PXD008703 | Pride | 2018-10-19

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Ear_RAWfiles.zip	Other
	Leaf_RAWfiles.zip	Other
	ear_phospho_heat_IWGSC.zip	Other
	ear_phospho_heat_wheatproteomeorg.zip	Other
	leaf_phospho_heat_IWGSC.zip	Other

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Publications

Temperature-induced changes in the wheat phosphoproteome reveal temperature-regulated interconversion of phosphoforms.

Vu Lam Dai LD Zhu Tingting T Verstraeten Inge I van de Cotte Brigitte B Gevaert Kris K De Smet Ive I

Journal of experimental botany 20180801 19

Wheat (Triticum ssp.) is one of the most important human food sources. However, this crop is very sensitive to temperature changes. Specifically, processes during wheat leaf, flower, and seed development and photosynthesis, which all contribute to the yield of this crop, are affected by high temperature. While this has to some extent been investigated on physiological, developmental, and molecular levels, very little is known about early signalling events associated with an increase in temperatu ...[more]

PMID: 29939309

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