Proteomics

Dataset Information

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Nannochloropsis oceanica cultivations display phenotypic heterogeneity in lipid metabolism

ABSTRACT: By combination of FACS sorting based on lipid sensitive Nile Red staining and small cell number proteomics the occurrence of population heterogeneity was analyzed. First population heterogeneity was identified by Nile Red staining and FACS in N. oceanica cells grown in nitrogen replete and nitrogen deplete media. For each condition two subpopulations(+NP3,+NP4,-NP3,-NP4) were detected and three replicates were sorted. Using the gathered proteome data proteins were identified and quantified with the MaxQuant software. Based on these quantification results a two sample t-test was performed to show significant regulation between +NP3 vs. +NP4 or -NP3 vs. -NP4. Goal was to understand the biological reasons for differences in lipid production between subpopulations.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Nannochloropsis Oceanica Strain Imet1

SUBMITTER: Andreas Harst  

LAB HEAD: Dr. Ansgar Poetsch

PROVIDER: PXD008721 | Pride | 2019-02-15

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
20180118_Noceanica_subpopulations_-NP3Rep1.raw Raw
20180118_Noceanica_subpopulations_-NP3Rep1.zip Other
20180118_Noceanica_subpopulations_-NP3Rep2.raw Raw
20180118_Noceanica_subpopulations_-NP3Rep2.zip Other
20180118_Noceanica_subpopulations_-NP3Rep3.raw Raw
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Publications

Proteomic study uncovers molecular principles of single-cell-level phenotypic heterogeneity in lipid storage of <i>Nannochloropsis oceanica</i>.

Chen Chaoyun C   Harst Andreas A   You Wuxin W   Xu Jian J   Ning Kang K   Poetsch Ansgar A  

Biotechnology for biofuels 20190204

<h4>Background</h4><i>Nannochloropsis oceanica</i> belongs to a large group of photoautotrophic eukaryotic organisms that play important roles in fixation and cycling of atmospheric CO<sub>2</sub>. Its capability of storing solar energy and carbon dioxide in the form of triacylglycerol (TAG) of up to 60% of total weight under nitrogen deprivation stress sparked interest in its use for biofuel production. Phenotypes varying in lipid accumulation among an <i>N. oceanica</i> population can be discl  ...[more]

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