Proteomics

Dataset Information

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Exoproteome analysis of Staphylococcus aureus clinical isolates belonging to different clonal complexes (C22, CC8 and CC398)


ABSTRACT: Studies on S. aureus sub-populations revealed that genomes are well conserved between isolates from the same lineages despite geographic, temporal and selective diversity. However, variation of hundreds of genes can occur between isolates from different lineages and these genes could be involved in interaction with host components. In this study, we aimed to investigate the diversity of secreted virulence factors in human and zoonotic S. aureus isolates from different clonal complexes. We focused on the S. aureus clonal complexes (CC) 8 and CC22 as dominant human lineages, and CC398 as dominant livestock-associated MRSA (LA-MRSA) which is disseminating rapidly. To study the diversity of secreted virulence factors, we compared their extracellular proteomes using label-free LC-MS/MS analysis. A common protein database was created based on DNA sequencing data and PAN genome IDs.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Staphylococcus Aureus

SUBMITTER: Tobias Busche  

LAB HEAD: Haike Antelmann

PROVIDER: PXD008797 | Pride | 2018-09-11

REPOSITORIES: Pride

Dataset's files

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41_1_A.raw Raw
41_1_B.raw Raw
41_1_C.raw Raw
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Publications

Comparative Secretome Analyses of Human and Zoonotic <i>Staphylococcus aureus</i> Isolates CC8, CC22, and CC398.

Busche Tobias T   Hillion Mélanie M   Van Loi Vu V   Berg David D   Walther Birgit B   Semmler Torsten T   Strommenger Birgit B   Witte Wolfgang W   Cuny Christiane C   Mellmann Alexander A   Holmes Mark A MA   Kalinowski Jörn J   Adrian Lorenz L   Bernhardt Jörg J   Antelmann Haike H  

Molecular & cellular proteomics : MCP 20180910 12


The spread of methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) in the community, hospitals and in livestock is mediated by highly diverse virulence factors that include secreted toxins, superantigens, enzymes and surface-associated adhesins allowing host adaptation and colonization. Here, we combined proteogenomics, secretome and phenotype analyses to compare the secreted virulence factors in selected <i>S. aureus</i> isolates of the dominant human- and livestock-associated genetic line  ...[more]

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