Proteomics,Multiomics

Dataset Information

0

Analysis of extracelullar vesicles (EVs) protein cargo across the bovine estrous cycle


ABSTRACT: The success of the early reproductive events depends on an appropriate communication between gametes/embryos and the oviduct. Extracellular vesicles (EVs) contained in oviductal secretions have been suggested as new players in mediating this crucial cross-talk by transferring their cargo (e.g. proteins, mRNA and small ncRNA) from cell to cell. Therefore, the objective of the project was to analyze the protein content of EVs (exosomes and microvesicles) contained in the bovine oviductal fluid at different stages of the estrus cycle. For this purpose, pairs of oviducts with their attached ovaries were collected from cyclic cows at a local abattoir under different hormonal regulation along the estrous cycle. Oviducts were classified into one of the 4 stages of the estrous cycle according to the morphology of the corpus lutea(CL) and follicle populations: Stage 1 (S1): showed recently ovulated follicle (d 1 to 4 of estrous cycle); Stage 2 (S2): early luteal development with medium or large or both present (d 5 to 11); Stage 3 (S3): fully functional CL yellow or orange in color (d 11 to 17); or Stage 4 (S4): regressing CL with little vasculature and a large preovulatory follicle present (d 18 to 20). EVs were collected by ultracentrifugation and prepared for mass spectrometry analysis.

OTHER RELATED OMICS DATASETS IN: GSE110444

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Oviductal Fluid

SUBMITTER: Lucie Combes-Soia  

LAB HEAD: Carmen Alminana Brines

PROVIDER: PXD008851 | Pride | 2018-07-31

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
16-0604_CarmenS3_1_A_ORBI2040.msf Msf
16-0604_CarmenS3_1_A_ORBI2040.raw Raw
16-0604_Carmen_S1_2_C_ORB2041.msf Msf
16-0604_Carmen_S1_2_C_ORB2041.raw Raw
16-0704_Carmen_EXO_S4_3_c_ORBI779.msf Msf
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Publications

Deciphering the oviductal extracellular vesicles content across the estrous cycle: implications for the gametes-oviduct interactions and the environment of the potential embryo.

Almiñana C C   Tsikis G G   Labas V V   Uzbekov R R   da Silveira J C JC   Bauersachs S S   Mermillod P P  

BMC genomics 20180822 1


<h4>Background</h4>The success of early reproductive events depends on an appropriate communication between gametes/embryos and the oviduct. Extracellular vesicles (EVs) contained in oviductal secretions have been suggested as new players in mediating this crucial cross-talk by transferring their cargo (proteins, mRNA and small ncRNA) from cell to cell. However, little is known about the oviductal EVs (oEVS) composition and their implications in the reproductive success. The aim of the study was  ...[more]

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