Proteomics

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Deep proteogenomics of Bacillus subtilis provides insights into function and evolution of bacterial organism

ABSTRACT: "Bacillus subtilis is an aerobic, endospore forming, rod-shaped Gram-positive bacterium. It has a relatively small genome of 4,215,606 bp with 4,197 protein coding genes. It is considered a model organism to study natural phenomena, such as chromosome replication, sporulation or swarming motility. Furthermore, many bacterial pathogens are closely related to B. subtilis, making it a highly significant system for research on potential targets for drug therapeutics. Although B. subtilis is among the best characterised bacterial systems, many of its gene products are still non-annotated, completely uncharacterised and/or their post-translational landscape is unknown. In the current study we aim at broadening the available information on Bacillus subtilis by providing a comprehensive resource for the microbial community encompassing a wide array of information at the genome, proteome, phosphoproteome and acetylome level."

INSTRUMENT(S): LTQ Orbitrap XL, LTQ Orbitrap Elite

ORGANISM(S): Bacillus Subtilis Subsp. Subtilis Str. 168

SUBMITTER: Nicolas Nalpas  

LAB HEAD: Prof. Dr. Boris Macek

PROVIDER: PXD008860 | Pride | 2018-11-28

REPOSITORIES: Pride

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Dataset's files

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Action DRS
01052012_BoSo_First_transition_Bacillus_SUPERSILAC_CID_test.raw Raw
01052012_BoSo_First_transition_Bacillus_SUPERSILAC_CID_test_Dec27tune.raw Raw
01052012_BoSo_First_transition_Bacillus_SUPERSILAC_HCD_test.raw Raw
01052012_BoSo_First_transition_Bacillus_SUPERSILAC_HCD_test_Dec27tune.raw Raw
01072012_BoSo_BSubtilis_SUPER_SILAC_InGel_ES_Slice01.raw Raw
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Publications

In-depth analysis of Bacillus subtilis proteome identifies new ORFs and traces the evolutionary history of modified proteins.

Ravikumar Vaishnavi V   Nalpas Nicolas C NC   Anselm Viktoria V   Krug Karsten K   Lenuzzi Maša M   Šestak Martin Sebastijan MS   Domazet-Lošo Tomislav T   Mijakovic Ivan I   Macek Boris B  

Scientific reports 20181122 1

Bacillus subtilis is a sporulating Gram-positive bacterium widely used in basic research and biotechnology. Despite being one of the best-characterized bacterial model organism, recent proteomics studies identified only about 50% of its theoretical protein count. Here we combined several hundred MS measurements to obtain a comprehensive map of the proteome, phosphoproteome and acetylome of B. subtilis grown at 37 °C in minimal medium. We covered 75% of the theoretical proteome (3,159 proteins),  ...[more]

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