Dataset Information

Comparative proteomic analysis of susceptible and resistance maize lines to southern corn rust

ABSTRACT: Southern corn rust (SCR) is one of destructive diseases in maize caused by Puccinia polysora Undrew. (P. polysara), widely occurring in warm-temperate and tropical regions globally. To identify candidate SCR resistance-related proteins and understand the molecular mechanism underlaying the maize and P. polysara interaction, comparative proteomic analysis of susceptible and resistance maize lines was performed. A total of 6,612 proteins were successfully identified using an iTRAQ-based proteomic approach. Fold changes and statistical analysis demonstrated that 687 proteins increased and 802 proteins decreased in the resistant line, while 571 increased and 464 decreased in the susceptible line. One remorin protein, namely ZmREM1.3 (B4G1B0), was significantly induced by SCR in the resistant genotype, while decreased in susceptible genotype after P. polysara infection. Plant-specific remorin proteins have been shown to play important roles during microbial infection and plant signaling processes. Transgenic analysis showed that overexpression of ZmREM1.3 in maize confers enhanced resistance to the biotrophic fungal pathogen SCR. Upon pathogen challenge, the ZmREM1.3-overexpressing plants accumulated higher levels of defense hormones, SA and JA. Moreover, stronger induction of defense gene expression was also observed in ZmREM1.3-overexpressing maize plants in response to SCR infection. Taken together, our results support that ZmREM1.3 plays a positive role in regulating the maize defense against SCR likely through SA/JA-mediated defense signaling pathways. This is the first attempt for large scale analysis of the molecular mechanisms underlaying the maize and P. polysara interaction at the proteomic level, and the first evidence for remorin protein family in resistant to fungal disease.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Zea Mays (maize)

TISSUE(S): Leaf

SUBMITTER: Shunxi Wang

LAB HEAD: Liuji Wu

PROVIDER: PXD008904 | Pride | 2019-04-18

REPOSITORIES: Pride

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Comparative proteomics combined with analyses of transgenic plants reveal ZmREM1.3 mediates maize resistance to southern corn rust.

Wang Shunxi S Chen Zan Z Tian Lei L Ding Yezhang Y Zhang Jun J Zhou Jinlong J Liu Ping P Chen Yanhui Y Wu Liuji L

Plant biotechnology journal 20190423 11

Southern corn rust (SCR), which is a destructive disease caused by Puccinia polysora Underw. (P. polysora), commonly occurs in warm-temperate and tropical regions. To identify candidate proteins related to SCR resistance and characterize the molecular mechanisms underlying the maize-P. polysora interaction, a comparative proteomic analysis of susceptible and resistant maize lines was performed. Statistical analyses revealed 1489 differentially abundant proteins in the resistant line, as well as ...[more]

PMID: 30972847

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