Dataset Information

Online Weak Cation Exchange-HILIC × Reversed Phase LC UVPD-HRMS of intact HeLa Histones

ABSTRACT: Intact HeLa core Histones were analyzed using an online two-dimensional liquid chromatographic separation as well as one dimensional LC (RPLC and WCX-HILIC) coupled with UVPD-MS

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Andrea Gargano

LAB HEAD: Ljiljana Paša-Tolić

PROVIDER: PXD008978 | Pride | 2018-09-19

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	2016_11_20_WCX_HILICaxmRPLC_UVPD_HeLaHistone_245_1_5ug_R1.raw	Raw
	2016_11_20_WCX_HILICaxmRPLC_UVPD_HeLaHistone_245_1_5ug_R2.raw	Raw
	2016_11_20_WCX_HILICaxmRPLC_UVPD_HeLaHistone_245_1_5ug_R3.raw	Raw
	2016_11_21_WCX_HILIC_UVPD_HeLaHistone_245_1_5ug.raw	Raw
	Listofproteoformidentified_CScore_Table2.xlsx	Xlsx

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Publications

Increasing the Separation Capacity of Intact Histone Proteoforms Chromatography Coupling Online Weak Cation Exchange-HILIC to Reversed Phase LC UVPD-HRMS.

Gargano Andrea F G AFG Shaw Jared B JB Zhou Mowei M Wilkins Christopher S CS Fillmore Thomas L TL Moore Ronald J RJ Somsen Govert W GW Paša-Tolić Ljiljana L

Journal of proteome research 20181008 11

Top-down proteomics is an emerging analytical strategy to characterize combinatorial protein post-translational modifications (PTMs). However, sample complexity and small mass differences between chemically closely related proteoforms often limit the resolution attainable by separations employing a single liquid chromatographic (LC) principle. In particular, for ultramodified proteins like histones, extensive and time-consuming fractionation is needed to achieve deep proteoform coverage. Herein, ...[more]

PMID: 30226781

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