Proteomics

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Proteomic analysis of human sclera tissue using high resolution mass spectrometry

ABSTRACT: The sclera is white, tough outer covering, known for providing structural support to the eyeball and the intraocular contents. Any abnormality in sclera results in disorders such as conjunctivitis, episcleritis, scleritis and hence a proper investigation will help in preventing the same. In the current study, we carried out an in-depth proteomic analysis of human sclera in which the proteins were extracted from the sclera tissue and subjected to both in-gel and in-solution digestion using trypsin as the proteolytic enzyme. The peptides were analyzed using Orbitrap Fusion Tribrid mass spectrometerand the data acquired was searched against Human RefSeq 75 protein database using SEQUEST HT and Mascot which resulted in the identification of 4,493 non-redundant proteins.The outcome of this study will facilitate in discovery of potential candidate biomarkers aiding in curbing the scleral disorders.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Keshava Prasad T. S.  

LAB HEAD: T. S. Keshava Prasad

PROVIDER: PXD008999 | Pride | 2019-02-21

REPOSITORIES: Pride

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Sclera_IG.msf Msf
Sclera_IG_1.raw Raw
Sclera_IG_10.raw Raw
Sclera_IG_11.raw Raw
Sclera_IG_12.raw Raw
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Publications

Eye disorders and resulting visual loss are major public health problems affecting millions of people worldwide. In this context, the sclera is an opaque, thick outer coat covering more than 80% of the eye, and essential in maintaining the shape of the eye and protecting the intraocular contents against infection and the external environment. Despite efforts undertaken to decipher the scleral proteome, the functional and structural picture of the sclera still remain elusive. Recently, proteomics  ...[more]

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