Proteomics

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Metaproteome analysis of hydrothermal vent chimney biofilms

ABSTRACT: At hydrothermal vent sites, chimneys consisting of sulfides, sulfates, and oxides are formed upon contact of reduced hydrothermal fluids with oxygenated seawater. The walls and surfaces of these chimneys are an important habitat for vent-associated microorganisms. We used community proteogenomics to investigate and compare the composition and in situ protein expression of microbial communities colonizing two actively venting hydrothermal chimneys from the Manus Basin back-arc spreading center (Papua New Guinea).

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Archaea Bacteria Viruses Eukaryota (eucaryotes)

SUBMITTER: Stephanie Markert  

LAB HEAD: Stephanie Markert

PROVIDER: PXD009105 | Pride | 2018-04-27

REPOSITORIES: Pride

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121026_V2_P6_AO_SM_Chimney_1a_10.raw Raw
121026_V2_P6_AO_SM_Chimney_1a_1b.raw Raw
121026_V2_P6_AO_SM_Chimney_1a_2.raw Raw
121026_V2_P6_AO_SM_Chimney_1a_3.raw Raw
121026_V2_P6_AO_SM_Chimney_1a_4.raw Raw
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Publications

Metaproteogenomic Profiling of Microbial Communities Colonizing Actively Venting Hydrothermal Chimneys.

Pjevac Petra P   Meier Dimitri V DV   Markert Stephanie S   Hentschker Christian C   Schweder Thomas T   Becher Dörte D   Gruber-Vodicka Harald R HR   Richter Michael M   Bach Wolfgang W   Amann Rudolf R   Meyerdierks Anke A  

Frontiers in microbiology 20180406

At hydrothermal vent sites, chimneys consisting of sulfides, sulfates, and oxides are formed upon contact of reduced hydrothermal fluids with oxygenated seawater. The walls and surfaces of these chimneys are an important habitat for vent-associated microorganisms. We used community proteogenomics to investigate and compare the composition, metabolic potential and relative <i>in situ</i> protein abundance of microbial communities colonizing two actively venting hydrothermal chimneys from the Manu  ...[more]

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