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PPARgamma regulates a cell-to-cell communication program in human methastatic melanoma cells

ABSTRACT: We mapped the proteins secreted by A375 melanoma cells in the presence or absence of the PPARagonist rosiglitazone.

INSTRUMENT(S): Orbitrap Fusion, Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Melanocyte, Skin

DISEASE(S): Melanoma

SUBMITTER: Manfredo Quadroni

LAB HEAD: Liliane Michalik

PROVIDER: PXD009108 | Pride | 2019-02-18

REPOSITORIES: Pride

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			Action	DRS
	20160526_Pich_8462_140min_2ul.raw	Raw
	20160526_Pich_8463_140min_2ul.raw	Raw
	20160706_Pich_8541_140min_2ul.raw	Raw
	20160706_Pich_8542_140min_2ul.raw	Raw
	20160819_Pich_8609_140min_2ul.raw	Raw

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Publications

Induction of Paracrine Signaling in Metastatic Melanoma Cells by PPARγ Agonist Rosiglitazone Activates Stromal Cells and Enhances Tumor Growth.

Pich Christine C Meylan Patrick P Mastelic-Gavillet Beatris B Nguyen Thanh Nhan TN Loyon Romain R Trang Bao Khanh BK Moser Hélène H Moret Catherine C Goepfert Christine C Hafner Jürg J Levesque Mitchell P MP Romero Pedro P Jandus Camilla C Michalik Liliane L

Cancer research 20180905 22

In addition to improving insulin sensitivity in type 2 diabetes, the thiazolidinedione family of compounds and the pharmacologic activation of their best-characterized target PPARγ have been proposed as a therapeutic option for cancer treatment. In this study, we reveal a new mode of action for the thiazolidinedione rosiglitazone that can contribute to tumorigenesis. Rosiglitazone activated a tumorigenic paracrine communication program in a subset of human melanoma cells that involves the secret ...[more]

PMID: 30185551

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Project description:We assessed the impact of Endothelial Focal Adhesion kinase (ECFAK) knockout, using primary lung endothelial cells isolated (at P10-P14) from a FAK flox/flox mice crossed with mice expressing a tamoxifen-inducible Cre(iCreERT2) that is driven by endothelial cell (EC)-selective platelet-derived growth factor subunit B (Pdgfb) promoter (Pdgfb-iCreERT2) (Tavora et al., 2010). To induce EC restricted FAK deletion, we injected 4-hydroxytamoxifen (4-OHT) from postnatal day 1 (P1) to P2. Molecular signaling events modulated by ECFAK loss, with or without ex vivo VEGF stimulation, were then examined by phospho and total quantitative proteomics analysis using 6plex isobaric tandem mass tagging multiplexing (TMT). In total, three experiments were performed (a an initial pilot experiment not included in this study, followed by two main biological replicates included - i.e. experiments 2, and 3). The following 4 TMT channels were utilised in each experiment: TMT-126: WT-mock(PBS) stimulated TMT-127: WT-Vegf stimulated TMT128: FAK KO-mock (PBS)stimulated TMT 129:FAK KO-Vegf stimulated

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The Arabidopsis TETRATRICOPEPTIDE-REPEAT THIOREDOXIN-LIKE (TTL) family members are involved in root system formation via their interaction with cytoskeleton and cell wall remodeling

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