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PPARgamma regulates a cell-to-cell communication program in human methastatic melanoma cells

ABSTRACT: We mapped the proteins secreted by A375 melanoma cells in the presence or absence of the PPARagonist rosiglitazone.

INSTRUMENT(S): Orbitrap Fusion, Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Melanocyte, Skin

DISEASE(S): Melanoma

SUBMITTER: Manfredo Quadroni

LAB HEAD: Liliane Michalik

PROVIDER: PXD009108 | Pride | 2019-02-18

REPOSITORIES: Pride

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			Action	DRS
	20160526_Pich_8462_140min_2ul.raw	Raw
	20160526_Pich_8463_140min_2ul.raw	Raw
	20160706_Pich_8541_140min_2ul.raw	Raw
	20160706_Pich_8542_140min_2ul.raw	Raw
	20160819_Pich_8609_140min_2ul.raw	Raw

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Publications

Induction of Paracrine Signaling in Metastatic Melanoma Cells by PPARγ Agonist Rosiglitazone Activates Stromal Cells and Enhances Tumor Growth.

Pich Christine C Meylan Patrick P Mastelic-Gavillet Beatris B Nguyen Thanh Nhan TN Loyon Romain R Trang Bao Khanh BK Moser Hélène H Moret Catherine C Goepfert Christine C Hafner Jürg J Levesque Mitchell P MP Romero Pedro P Jandus Camilla C Michalik Liliane L

Cancer research 20180905 22

In addition to improving insulin sensitivity in type 2 diabetes, the thiazolidinedione family of compounds and the pharmacologic activation of their best-characterized target PPARγ have been proposed as a therapeutic option for cancer treatment. In this study, we reveal a new mode of action for the thiazolidinedione rosiglitazone that can contribute to tumorigenesis. Rosiglitazone activated a tumorigenic paracrine communication program in a subset of human melanoma cells that involves the secret ...[more]

PMID: 30185551

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Project description:We assessed the impact of Endothelial Focal Adhesion kinase (ECFAK) knockout, using primary lung endothelial cells isolated (at P10-P14) from a FAK flox/flox mice crossed with mice expressing a tamoxifen-inducible Cre(iCreERT2) that is driven by endothelial cell (EC)-selective platelet-derived growth factor subunit B (Pdgfb) promoter (Pdgfb-iCreERT2) (Tavora et al., 2010). To induce EC restricted FAK deletion, we injected 4-hydroxytamoxifen (4-OHT) from postnatal day 1 (P1) to P2. Molecular signaling events modulated by ECFAK loss, with or without ex vivo VEGF stimulation, were then examined by phospho and total quantitative proteomics analysis using 6plex isobaric tandem mass tagging multiplexing (TMT). In total, three experiments were performed (a an initial pilot experiment not included in this study, followed by two main biological replicates included - i.e. experiments 2, and 3). The following 4 TMT channels were utilised in each experiment: TMT-126: WT-mock(PBS) stimulated TMT-127: WT-Vegf stimulated TMT128: FAK KO-mock (PBS)stimulated TMT 129:FAK KO-Vegf stimulated

2022-04-13 | PXD030824 | Pride

The Arabidopsis TETRATRICOPEPTIDE-REPEAT THIOREDOXIN-LIKE (TTL) family members are involved in root system formation via their interaction with cytoskeleton and cell wall remodeling

Project description:Lateral roots are essential components of the plant edaphic interface; contributing to water and nutrient uptake, biotic and abiotic interactions, stress survival, and plant anchorage. We have identified the TETRATRICOPEPTIDE-REPEAT THIOREDOXIN-LIKE 3 (TTL3) gene as being related to lateral root emergence and later development. Loss of function of TTL3 leads to a reduced number of emerged lateral roots due to delayed development of lateral root primordia. This trait is further enhanced in the triple mutant ttl1ttl3ttl4. TTL3 interacts with microtubules and endomembranes and is known to participate in the brassinosteroid signaling pathway. Both ttl3 and ttl1ttl3ttl4 mutants are less sensitive to brassinosteroid treatment in terms of lateral root formation and primary root growth. The ability of TTL3 to modulate biophysical properties of the cell wall was established under restrictive conditions of hyperosmotic stress and loss of root growth recovery, which was enhanced in ttl1ttl3ttl4. Timing and spatial distribution of TTL3 expression is consistent with its role in development of lateral root primordia before their emergence and subsequent growth of lateral roots. TTL3 emerged as a novel component of the root system morphogenesis regulatory network.

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2020-12-17 | PXD019708 | Pride

Secretome and intracellular proteome of eccE1 and mycP1 deletion mutants of Mycobacterium tuberculosis

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2019-12-16 | PXD012584 | Pride

Secretome and intracellular proteome of espB deletion mutant of Mycobacterium tuberculosis

Project description:Mycobacterium tuberculosis relies on the ESX-1 secretion system for survival, multiplication in and escape from macrophages. In this work we investigate the role of the EspB protein, encoded within the esx-1 gene cluster, in virulence and ESX-1 substrate secretion in M. tuberculosis H37Rv. Genetic, proteomic and immunological data show that, contrary to previous reports, an espB knock-out mutant is only partially attenuated in ex vivo infection models, where EsxA, EsxB and EspC antigen presentation is not affected, and secretes the major virulence factor EsxA. Additionally, we demonstrate that EspB does not require an intact and functional ESX-1 apparatus for being secreted in H37Rv and in M. bovis BCG, as opposed to other strains such as CDC1551 and Erdman, thereby suggesting that other ESX systems may be involved in the process. Overall our findings highlight unexplored differences in the secretion profiles of various mycobacterial strains and underscore the plasticity of ESX-dependent secretion in mycobacteria.

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Genome Wide Phosphoproteome Analysis of Zymomonas Mobilis under Anaerobic, Aerobic, and N2-Fixing Conditions

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