Dataset Information

Saci proteome starvation - Early Response of Sulfolobus acidocaldarius to Nutrient Limitation

ABSTRACT: We performed a comprehensive study to unravel the overall transcriptomic, proteomic and metabolic changes that take places over time when S. acidocaldarius cells are exposed to nutrient limitation.

OTHER RELATED OMICS DATASETS IN: GSE113716

INSTRUMENT(S): Q Exactive

ORGANISM(S): Sulfolobus Acidocaldarius Dsm 639

SUBMITTER: Trong Khoa Pham

LAB HEAD: Trong K Pham

PROVIDER: PXD009111 | Pride | 2018-12-21

REPOSITORIES: pride

ACCESS DATA

Dataset's files

Source:

Items per page:

1 - 5 of 14

Publications

Early Response of <i>Sulfolobus acidocaldarius</i> to Nutrient Limitation.

Bischof Lisa F LF Haurat M Florencia MF Hoffmann Lena L Albersmeier Andreas A Wolf Jacqueline J Neu Astrid A Pham Trong Khoa TK Albaum Stefan P SP Jakobi Tobias T Schouten Stefan S Neumann-Schaal Meina M Wright Phillip C PC Kalinowski Jörn J Siebers Bettina B Albers Sonja-Verena SV

Frontiers in microbiology 20190110

In natural environments microorganisms encounter extreme changes in temperature, pH, osmolarities and nutrient availability. The stress response of many bacterial species has been described in detail, however, knowledge in Archaea is limited. Here, we describe the cellular response triggered by nutrient limitation in the thermoacidophilic crenarchaeon <i>Sulfolobus acidocaldarius</i>. We measured changes in gene transcription and protein abundance upon nutrient depletion up to 4 h after initiati ...[more]

PMID: 30687244

Similar Datasets

Project description:Mounting evidence demonstrates that nutritional environment can alter pathogen drug sensitivity. While the rich media used for in vitro culture contains supraphysiological nutrient concentrations, pathogens encounter a relatively restrictive environment in vivo. We assessed the effect of nutrient limitation on the protozoan parasite that causes malaria and demonstrated that short-term growth under physiologically relevant mild nutrient stress (or "metabolic priming") triggers increased tolerance of a potent antimalarial drug. We observed beneficial effects using both short-term survival assays and longer-term proliferation studies, where metabolic priming increases parasite survival to a level previously defined as resistant (>1% survival). We performed these assessments by either decreasing single nutrients that have distinct roles in metabolism or using a media formulation that simulates the human plasma environment. We determined that priming-induced tolerance was restricted to parasites that had newly invaded the host red blood cell, but the effect was not dependent on genetic background. The molecular mechanisms of this intrinsic effect mimic aspects of genetic tolerance, including translational repression and protein export. This finding suggests that regardless of the impact on survival rates, environmental stress could stimulate changes that ultimately directly contribute to drug tolerance. Because metabolic stress is likely to occur more frequently in vivo compared to the stable in vitro environment, priming-induced drug tolerance has ramifications for how in vitro results translate to in vivo studies. Improving our understanding of how pathogens adjust their metabolism to impact survival of current and future drugs is an important avenue of research to slow the evolution of resistance. IMPORTANCE There is a dire need for effective treatments against microbial pathogens. Yet, the continuing emergence of drug resistance necessitates a deeper knowledge of how pathogens respond to treatments. We have long appreciated the contribution of genetic evolution to drug resistance, but transient metabolic changes that arise in response to environmental factors are less recognized. Here, we demonstrate that short-term growth of malaria parasites in a nutrient-limiting environment triggers cellular changes that lead to better survival of drug treatment. We found that these strategies are similar to those employed by drug-tolerant parasites, which suggests that starvation "primes" parasites to survive and potentially evolve resistance. Since the environment of the human host is relatively nutrient restrictive compared to growth conditions in standard laboratory culture, this discovery highlights the important connections among nutrient levels, protective cellular pathways, and resistance evolution.

			Action	DRS
	Proteomics_PRIDE_Results.xlsx	Xlsx
	Saci_Stavation_01.raw	Raw
	Saci_Stavation_02.raw	Raw
	Saci_Stavation_03.raw	Raw
	Saci_Stavation_04.raw	Raw