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Transcriptomic and Proteomic Insight into the Mechanism of Cyclooctasulfur- versus Thiosulfate-Oxidation by the Chemolithoautotroph Sulfurimonas denitrificans

ABSTRACT: Chemoautotrophic bacteria belonging to the genus Sulfurimonas in the class Campylobacteria (formerly classified as Epsilonproteobacteria) play a key role in the sulfur cycle in a variety of oxygen-deficient or –limited and sulfide-rich marine and terrestrial environments. Previously, they were identified as key players in the turnover of zero-valence sulfur, a central intermediate in the marine sulfur cycle, and S. denitrificans was further shown to be able to oxidize cyclooctasulfur. However, at present the mechanism involved in the activation and metabolism of cyclooctasulfur is not known. To this end, we assessed the transcriptome and proteome of S. denitrificans grown with either thiosulfate or cyclooctasulfur as the electron donor. While the overall profiles under the two growth conditions were rather similar, distinct differences were observed that could be attributed to the utilization of cyclooctasulfur. This included a higher abundance of expressed genes and proteins related to attachment in the presence of cyclooctasulfur and the differential expression of the sulfur-oxidation multienzyme complex (SOX). S. denitrificans uses the SOX system for the oxidation of reduced sulfur compounds, including two copies of the sulfur-binding SoxYZ proteins, encoded in two gene clusters: soxABXYZ1 and soxCDYZ2. While the proteins of both operons of the SOX system were detected in the presence of thiosulfate, only proteins of the soxCDYZ2 operon were detected when grown with cylcooctasulfur. Based on these findings a model for the oxidation of cylcooctasulfur is being proposed that might also apply to other Campylobacteria that share the same arrangement of the SOX system. Our results have implications for interpreting metatranscriptomic and -proteomic data and for the observed high level of diversification of soxYZ2 among sulfur-oxidizing Campylobacteria.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Sulfurimonas Denitrificans

SUBMITTER: Florian Goetz

LAB HEAD: Stefan M. Sievert

PROVIDER: PXD009127 | Pride | 2019-07-22

REPOSITORIES: Pride

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			Action	DRS
	150608_O2_P1_SG_StM_FG_S8-1_1.raw	Raw
	150608_O2_P1_SG_StM_FG_S8-1_10.raw	Raw
	150608_O2_P1_SG_StM_FG_S8-1_2.raw	Raw
	150608_O2_P1_SG_StM_FG_S8-1_3.raw	Raw
	150608_O2_P1_SG_StM_FG_S8-1_4.raw	Raw

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Transcriptomic and proteomic insight into the mechanism of cyclooctasulfur- versus thiosulfate-oxidation by the chemolithoautotroph Sulfurimonas denitrificans.

Götz Florian F Pjevac Petra P Markert Stephanie S McNichol Jesse J Becher Dörte D Schweder Thomas T Mussmann Marc M Sievert Stefan M SM

Environmental microbiology 20181126 1

Chemoautotrophic bacteria belonging to the genus Sulfurimonas (class Campylobacteria) were previously identified as key players in the turnover of zero-valence sulfur, a central intermediate in the marine sulfur cycle. S. denitrificans was further shown to be able to oxidize cyclooctasulfur (S<sub>8</sub> ). However, at present the mechanism of activation and metabolism of cyclooctasulfur is not known. Here, we assessed the transcriptome and proteome of S. denitrificans grown with either thiosul ...[more]

PMID: 30362214

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