Dataset Information

Physiological and proteomic changes in soft wheat seeds during accelerated aging

ABSTRACT: Wheat (Triticum aestivum L.), one of the most important crops in the world, is a staple food used for making flour, noodles, alcoholic beverages, biofuel and a variety of other products. In wheat, kernel texture and biochemical composition vary with different hardnesses. Previous study of our research group indicated that physiological properties changes including germination ratio, and physiological enzymatic activities in wheat with different hardnesses were different. A comparative proteomic analysis of soft and hard wheat embryos by our research group identified more proteins associated with anti-resistance in soft wheat than that in hard wheat. Additionally, quantitative proteomic analysis of medium-hard wheat ‘Aikang58’ seeds showed that 162 differentially expressed proteins (DEPs) participated in metabolism, energy supply, and defense/stress responses were identified during artificial ageing were identified in our previous research. However, the dynamic physiological and quantitative proteomic changes in soft wheat seeds during accelerated aging remain unclear, thus need to be elucidated. In this work, we conducted the first Tandem Mass Tag (TMT)-based dynamic quantitative proteomic analysis of elite Chinese soft wheat cultivar ‘Yangmai 15’ seeds during artificial ageing. The uncovered differentially expressed proteins (DEPs) with different functions might provide new insights into the comprehensive understanding of deterioration in soft wheat seeds.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Triticum Aestivum (wheat)

TISSUE(S): Whole Body

SUBMITTER: xiaomei li

LAB HEAD: Yuansen Hu

PROVIDER: PXD009156 | Pride | 2018-10-08

REPOSITORIES: pride

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Dynamic proteomic changes in soft wheat seeds during accelerated ageing.

Lv Yangyong Y Tian Pingping P Zhang Shuaibing S Wang Jinshui J Hu Yuansen Y

PeerJ 20181102

Previous research demonstrated that soft wheat cultivars have better post-harvest storage tolerance than harder cultivars during accelerated ageing. To better understand this phenomenon, a tandem mass tag-based quantitative proteomic analysis of soft wheat seeds was performed at different storage times during accelerated ageing (germination ratios of 97%, 45%, 28%, and 6%). A total of 1,010 proteins were differentially regulated, of which 519 and 491 were up- and downregulated, respectively. Mos ...[more]

PMID: 30405971

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