Proteomics

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Drosophila insulin-like peptides DILP2 and DILP5 stimulation global phosphoproteome


ABSTRACT: Insulin and IGF signaling (IIS) is a complex system that controls diverse processes including growth, development, metabolism, stress responses and aging. Drosophila melanogaster IIS is propagated by eight Drosophila insulin-like peptides (DILPs), homologues of both mammalian insulin and IGFs, with various spatiotemporal expression patterns and functions. DILPs 1-7 are thought to act through a single Drosophila insulin/IGF receptor, InR, but it is unclear how the DILPs thereby mediate a range of physiological phenotypes. We determined the distinct cell signaling effects of DILP2 and DILP5 stimulation upon Drosophila S2 cells. DILP2 and DILP5 induced similar transcriptional patterns, but differed in signal transduction kinetics. DILP5 induced sustained phosphorylation of Akt, while DILP2 produced acute, transient Akt phosphorylation. Accordingly, we used phosphoproteomic analysis to identify distinct patterns of non-genomic signaling induced by DILP2 and DILP5. Across all treatments and replicates, 5250 unique phosphopeptides were identified, representing 1575 proteins. Among these peptides, DILP2, but not DILP5, dephosphorylated Ser15 on glycogen phosphorylase (GlyP), and DILP2, but not DILP5, was subsequently shown to repress enzymatic GlyP activity in S2 cells. The functional consequences of this difference were evaluated in adult Drosophila dilp mutants: dilp2 null adults have elevated GlyP enzymatic activity relative to wildtype, while dilp5 mutants have reduced GlyP activity.

OTHER RELATED OMICS DATASETS IN: PRJNA437433

INSTRUMENT(S): Q Exactive

ORGANISM(S): Drosophila Melanogaster Drosophila Melanogaster (fruit Fly)

TISSUE(S): Cell Culture

SUBMITTER: Stephanie Post  

LAB HEAD: Marc Tatar

PROVIDER: PXD009240 | Pride | 2018-10-19

REPOSITORIES: Pride

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2-10A.msf Msf
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2-10C.msf Msf
2-30A.msf Msf
2-30B.msf Msf
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Publications

<i>Drosophila</i> Insulin-Like Peptides DILP2 and DILP5 Differentially Stimulate Cell Signaling and Glycogen Phosphorylase to Regulate Longevity.

Post Stephanie S   Karashchuk Galina G   Wade John D JD   Sajid Waseem W   De Meyts Pierre P   Tatar Marc M  

Frontiers in endocrinology 20180528


Insulin and IGF signaling (IIS) is a complex system that controls diverse processes including growth, development, metabolism, stress responses, and aging. <i>Drosophila melanogaster</i> IIS is propagated by eight <i>Drosophila</i> insulin-like peptides (DILPs), homologs of both mammalian insulin and IGFs, with various spatiotemporal expression patterns and functions. DILPs 1-7 are thought to act through a single <i>Drosophila</i> insulin/IGF receptor, InR, but it is unclear how the DILPs thereb  ...[more]

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