Proteomics

Dataset Information

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Proteomic insights into ulvan degradation by the marine Flavobacterium Formosa agariphila


ABSTRACT: The marine Flavobacterium Formosa agariphila KMM 3901T is able to use a broad range of different carbohydrates as growth substrates. This is reflected in the strain’s repertoire of 13 polysaccharide utilization loci (PUL) in total. One PUL – termed as PUL H – is responsible for ulvan degradation, which is a widely distributed, algal-derived polysaccharide. The PUL comprises almost 40 genes, coding for transporters, lyases, glycoside hydrolases or sulfatases, among others. These proteins catalyse the breakdown of ulvan or the uptake of degradation products. A combined application of isotope labeling, subcellular protein fractionation and quantitative proteomics revealed that corresponding PUL encoded proteins were substrate specific up-regulated in ulvan-cultivated cells. The sulphated polysaccharide ulvan also induced the specific expression of proteins necessary for subsequent monosaccharide degradation. Compared to a control (fructose-cultivated cells), expression of PUL H additionally responded to rhamnose, a basic component of ulvan, indicating that this monosaccharide might signal ulvan availability in the environment. Our proteome analyses proofed a substrate specific expression of proteins involved in ulvan utilization and allowed us to deduce a comprehensive degradation pathway for this complex marine polysaccharide.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Formosa Agariphila Kmm 3901

SUBMITTER: Thomas Sura  

LAB HEAD: Dörte Becher

PROVIDER: PXD009299 | Pride | 2019-07-25

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
170406_O2_P7_SG_StM_Ulvan1.raw Raw
170406_O2_P7_SG_StM_Ulvan2.raw Raw
170406_O2_P7_SG_StM_Ulvan3.raw Raw
170412_O2_P7_SG_StM_U1_1.raw Raw
170412_O2_P7_SG_StM_U1_10.raw Raw
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Publications


Marine seaweeds increasingly grow into extensive algal blooms, which are detrimental to coastal ecosystems, tourism and aquaculture. However, algal biomass is also emerging as a sustainable raw material for the bioeconomy. The potential exploitation of algae is hindered by our limited knowledge of the microbial pathways-and hence the distinct biochemical functions of the enzymes involved-that convert algal polysaccharides into oligo- and monosaccharides. Understanding these processes would be es  ...[more]

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