Dataset Information

Contagious Caprine Pleuropneumonia vaccine

ABSTRACT: A tandem mass spectrometry approach was used to identify the mycoplasma antigen present in reference samples and in commercial CCPP vaccines. The same approach was used to quantify the relative amounts of mycoplasma antigen and residual proteins originating from the production medium.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mycoplasma Sp. Enrichment Culture

SUBMITTER: Jean ARMENGAUD

LAB HEAD: Jean Armengaud

PROVIDER: PXD009387 | Pride | 2018-07-05

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

Items per page:

1 - 5 of 73

Publications

Improving Quality Control of Contagious Caprine Pleuropneumonia Vaccine with Tandem Mass Spectrometry.

Thiaucourt François F Pible Olivier O Miotello Guylaine G Nwankpa Nick N Armengaud Jean J

Proteomics 20180820 17

Vaccines to protect livestock against contagious caprine pleuropneumonia (CCPP) consist of inactivated, adjuvanted antigens. Quality control of these vaccines is challenging as total protein quantification provides no indication of protein identity or purity, and culture is not an option. Here, a tandem mass spectrometry approach is used to identify the mycoplasma antigen contained in reference samples and in commercial CCPP vaccines. By the same approach, the relative amounts of mycoplasma anti ...[more]

PMID: 29911326

Similar Datasets

Project description:Kamminga2017 - Metabolic model of Mycoplasma hyopneumoniae growth This model is described in the article: Metabolic modeling of energy balances in Mycoplasma hyopneumoniae shows that pyruvate addition increases growth rate. Kamminga T, Slagman SJ, Bijlsma JJE, Martins Dos Santos VAP, Suarez-Diez M, Schaap PJ. Biotechnol. Bioeng. 2017 Jun; : Abstract: Mycoplasma hyopneumoniae is cultured on large-scale to produce antigen for inactivated whole-cell vaccines against respiratory disease in pigs. However, the fastidious nutrient requirements of this minimal bacterium and the low growth rate make it challenging to reach sufficient biomass yield for antigen production. In this study, we sequenced the genome of M. hyopneumoniae strain 11 and constructed a high quality constraint-based genome-scale metabolic model of 284 chemical reactions and 298 metabolites. We validated the model with time-series data of duplicate fermentation cultures to aim for an integrated model describing the dynamic profiles measured in fermentations. The model predicted that 84% of cellular energy in a standard M. hyopneumoniae cultivation was used for non-growth associated maintenance and only 16% of cellular energy was used for growth and growth associated maintenance. Following a cycle of model-driven experimentation in dedicated fermentation experiments, we were able to increase the fraction of cellular energy used for growth through pyruvate addition to the medium. This increase in turn led to an increase in growth rate and a 2.3 times increase in the total biomass concentration reached after 3-4 days of fermentation, enhancing the productivity of the overall process. The model presented provides a solid basis to understand and further improve M. hyopneumoniae fermentation processes. Biotechnol. Bioeng. 2017;9999: 1-9. © 2017 Wiley Periodicals, Inc. This model is hosted on BioModels Database and identified by: MODEL1704250001. To cite BioModels Database, please use: BioModels Database: An enhanced, curated and annotated resource for published quantitative kinetic models. To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information.

			Action	DRS
	F208265.dat	Other
	F208265.mzid.gz	Mzid
	F208265.pride.mztab.gz	Mztab
	F208266.dat	Other
	F208266.mzid.gz	Mzid