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Mouse CDC73 AP-MS in AML - PAF1 complex interactions with SETDB1 mediate promoter H3K9 methylation and transcriptional repression of Hoxa9 and Meis1 in acute myeloid leukemia

ABSTRACT: Here we performed AP-MS experiments to find novel binding partners of CDC73 in mouse M1 AML cells. We also utilized a CDC73 mutant that does not support AML cell growth to determine functional consequences of gainin/ losing protein-protein interactions on mutation of this protein.

INSTRUMENT(S): Orbitrap Fusion ETD

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Suspension Culture, Blood Cell, Blood

DISEASE(S): Acute Leukemia

SUBMITTER: James Ropa

LAB HEAD: Andrew G Muntean

PROVIDER: PXD009439 | Pride | 2018-05-22

REPOSITORIES: Pride

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			Action	DRS
	CDC73_mutant_rep1.msf	Msf
	CDC73_mutant_rep1.raw	Raw
	CDC73_mutant_rep2.msf	Msf
	CDC73_mutant_rep2.raw	Raw
	CDC73_mutant_rep3.msf	Msf

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Publications

PAF1 complex interactions with SETDB1 mediate promoter H3K9 methylation and transcriptional repression of <i>Hoxa9</i> and <i>Meis1</i> in acute myeloid leukemia.

Ropa James J Saha Nirmalya N Chen Zhiling Z Serio Justin J Chen Wei W Mellacheruvu Dattatreya D Zhao Lili L Basrur Venkatesha V Nesvizhskii Alexey I AI Muntean Andrew G AG

Oncotarget 20180424 31

The Polymerase Associated Factor 1 complex (PAF1c) is an epigenetic co-modifying complex that directly contacts RNA polymerase II (RNAPII) and several epigenetic regulating proteins. Mutations, overexpression and loss of expression of subunits of the PAF1c are observed in various forms of cancer suggesting proper regulation is needed for cellular development. However, the biochemical interactions with the PAF1c that allow dynamic gene regulation are unclear. We and others have shown that the PAF ...[more]

PMID: 29774127

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