Proteomics

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Thermal proteome profiling in Escherichia coli

ABSTRACT: We adapted thermal proteome profiling (TPP) to study the thermostability of Escherichia coli proteins in vivo. We monitored the E. coli meltome and proteome at different growth phases, in a tolC knock-out mutant and after drug treatment.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human) Escherichia Coli

SUBMITTER: Andre Mateus  

LAB HEAD: Mikhail M. Savitski

PROVIDER: PXD009495 | Pride | 2018-07-09

REPOSITORIES: Pride

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Bert_S0871_AM_Ecoli_lysate_meltome_withMg_n1_R1_F1.raw Raw
Bert_S0871_AM_Ecoli_lysate_meltome_withMg_n1_R1_F2.raw Raw
Bert_S0871_AM_Ecoli_lysate_meltome_withMg_n1_R1_F3.raw Raw
Bert_S0871_AM_Ecoli_lysate_meltome_withMg_n1_R1_F4.raw Raw
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Publications

Thermal proteome profiling in bacteria: probing protein state <i>in vivo</i>.

Mateus André A   Bobonis Jacob J   Kurzawa Nils N   Stein Frank F   Helm Dominic D   Hevler Johannes J   Typas Athanasios A   Savitski Mikhail M MM  

Molecular systems biology 20180706 7

Increasing antibiotic resistance urges for new technologies for studying microbes and antimicrobial mechanism of action. We adapted thermal proteome profiling (TPP) to probe the thermostability of <i>Escherichia coli</i> proteins <i>in vivo</i><i>E. coli</i> had a more thermostable proteome than human cells, with protein thermostability depending on subcellular location-forming a high-to-low gradient from the cell surface to the cytoplasm. While subunits of protein complexes residing in one comp  ...[more]

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