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Protein complex identification in Arabidopsis thaliana root membranes through SEC-MS

ABSTRACT: With the information gathered through mass-shift- and correlation-analysis, we predict PPi on a proteomic scale and describe the results in form of a network. This experiment was done with 3 week old Arabidopsis thaliana wt seedlings grown in hydroponic culture (1/2 MS-Medium)

INSTRUMENT(S): Q Exactive

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Root

SUBMITTER: Max Gilbert

LAB HEAD: Waltraud Schulze

PROVIDER: PXD009511 | Pride | 2018-11-04

REPOSITORIES: Pride

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Publications

Global Identification of Protein Complexes within the Membrane Proteome of Arabidopsis Roots Using a SEC-MS Approach.

Gilbert Max M Schulze Waltraud X WX

Journal of proteome research 20181113 1

Biological processes consist of several consecutive and interacting steps as, for example, in signal transduction cascades or metabolic reaction chains. These processes are regulated by protein-protein interactions and the formation of larger protein complexes, which also occur within biological membranes. To gain a large-scale overview of complex-forming proteins and the composition of such complexes within the cellular membranes of Arabidopsis roots, we use the combination of size-exclusion ch ...[more]

PMID: 30370772

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