Proteomics

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Extended ZIKV-NS4B interactome (AP-MS/MS analysis of ZIKV interacting proteins)

ABSTRACT: To identify the cellular interactors of ZIKV-NS4B-interacting proteins and their differential composition upon ZIKV infection, individual cDNAs expressing FLAG-tagged variants of the following ZIKV-NS4B-interacting proteins were generated (GeneBank and UniProt Accession numbers are given in parenthesis): CEND1 (BC034732.1; Q8N111), CHP1 (BC031293; Q99653), CLN6 (BC010849.1; Q9NWW5), TMEM41b (BC035034.1; Q5BJD5), RBFOX2 (NM_001082579.1; O43251-6), BSG (BC009040.2;P35613). Lentiviruses expressing each of these proteins were used to transduce SK-N-BE2 cells, which were either mock-infected or infected with ZIKV (H/PF/2013, MOI=3), and 72 hours later subjected to AP-MS/MS to identify the respective cellular interactors.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Zika Virus Zikv/h. Sapiens/frenchpolynesia/10087pf/2013 Homo Sapiens (human)

TISSUE(S): Cell Culture, Neuroblast

SUBMITTER: Pietro Scaturro  

LAB HEAD: Andreas Pichlmair

PROVIDER: PXD009561 | Pride | 2018-09-03

REPOSITORIES: Pride

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20170124_QEp5_PiSc_SA_BSG_ZIKV_1.raw Raw
20170124_QEp5_PiSc_SA_BSG_ZIKV_2.raw Raw
20170124_QEp5_PiSc_SA_BSG_ZIKV_3.raw Raw
20170124_QEp5_PiSc_SA_BSG_ZIKV_4.raw Raw
20170124_QEp5_PiSc_SA_BSG_mock_1.raw Raw
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Publications

Zika virus (ZIKV) has recently emerged as a global health concern owing to its widespread diffusion and its association with severe neurological symptoms and microcephaly in newborns<sup>1</sup>. However, the molecular mechanisms that are responsible for the pathogenicity of ZIKV remain largely unknown. Here we use human neural progenitor cells and the neuronal cell line SK-N-BE2 in an integrated proteomics approach to characterize the cellular responses to viral infection at the proteome and ph  ...[more]

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