Proteomics

Dataset Information

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Toxoplasma GRA protein identification


ABSTRACT: Toxoplasma gondii is an opportunistic pathogen infecting humans and a variety of vertebrate animals. Secretory dense granule proteins (GRA) are important effectors that mediate host-parasite interactions and facilitate parasitism. Due to the wide host range, T. gondii needs diverse GRA proteins to accommodate parasite survival in different hosts. In this study, we used two proximity based protein labeling techniques to identify novel GRA proteins. Using GRA1 as bait, two transgenic strains expressing GRA1-BirA* or GRA1-APEX were used to biotinylate GRA candidate proteins. Using these methods, 90 proteins were identified by APEX based labeling and 56 by the BirA* approach. Twenty-three were identified by both techniques, among which 13 are known GRA proteins. In combination 98 novel GRA candidates were identified by these two approaches and 25 are known GRAs. The majority of them are only present in coccidian parasites and dispensable for parasite growth in vitro, they likely mediate interactions with hosts and play roles during animal infections.

INSTRUMENT(S): Q-Tof Ultima

ORGANISM(S): Toxoplasma

TISSUE(S): Cell Culture, Fibroblast

DISEASE(S): Toxoplasmosis

SUBMITTER: Bang Shen  

LAB HEAD: Bang Shen

PROVIDER: PXD009604 | Pride | 2018-11-02

REPOSITORIES: Pride

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Publications

Identification of Novel Dense-Granule Proteins in Toxoplasma gondii by Two Proximity-Based Biotinylation Approaches.

Pan Ming M   Li Mingjun M   Li Longjiao L   Song Yongle Y   Hou Lun L   Zhao Junlong J   Shen Bang B  

Journal of proteome research 20181026 1


Toxoplasma gondii is an opportunistic pathogen infecting humans and a variety of vertebrate animals. Secretory dense-granule proteins (GRAs) play diverse roles in the mediation of host-parasite interactions and facilitate parasitism, but many of them still remain to be identified. Here, we used two proximity-based protein labeling techniques to identify novel GRA proteins. Taking GRA1 as bait, transgenic strains expressing GRA1-BirA* or GRA1-APEX were constructed to biotinylate GRAs. Using these  ...[more]

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