Dataset Information

Photoferrotroph R. palustris TIE-1

ABSTRACT: The underlying adaptations required by anoxygenic phototrophs to oxidize Fe(II), a potential stressor, are not well constrained. We used quantitative proteomics to compare cells of the photoferrotroph Rhodopseudomonas palustris TIE-1 grown photoautotrophically with Fe(II) or H2, and photoheterotrophically with acetate. We observed unique proteome profiles for each condition with differences primarily driven by carbon source. Growth on Fe(II) was characterized by a response typical of iron homeostasis which included an increased abundance of proteins required for metal efflux (particularly copper), and decreased abundance of iron import proteins, including siderophore receptors, with no evidence of further stressors such as oxidative damage. This study suggests that the main challenge facing photoferrotrophs comes from limitations imposed by autotrophic growth and, once this challenge is overcome, iron stress can be mitigated using iron management mechanisms common to diverse bacteria.

INSTRUMENT(S): LTQ Orbitrap XL

ORGANISM(S): Rhodopseudomonas Palustris Tie-1

SUBMITTER: Nicolas Nalpas

LAB HEAD: Prof. Dr. Andreas Kappler

PROVIDER: PXD009629 | Pride | 2018-06-25

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	20160923_CO_0650AnKa_R02.raw	Raw
	20160923_CO_0650AnKa_R03.raw	Raw
	20160923_CO_0650AnKa_R04.raw	Raw
	20160923_CO_0650AnKa_R05.raw	Raw
	20160923_CO_0650AnKa_R06.raw	Raw

Items per page:

1 - 5 of 10

Publications

Proteome Response of a Metabolically Flexible Anoxygenic Phototroph to Fe(II) Oxidation.

Bryce Casey C Franz-Wachtel Mirita M Nalpas Nicolas C NC Miot Jennyfer J Benzerara Karim K Byrne James M JM Kleindienst Sara S Macek Boris B Kappler Andreas A

Applied and environmental microbiology 20180801 16

The oxidation of Fe(II) by anoxygenic photosynthetic bacteria was likely a key contributor to Earth's biosphere prior to the evolution of oxygenic photosynthesis and is still found in a diverse range of modern environments. All known phototrophic Fe(II) oxidizers can utilize a wide range of substrates, thus making them very metabolically flexible. However, the underlying adaptations required to oxidize Fe(II), a potential stressor, are not completely understood. We used a combination of quantita ...[more]

PMID: 29915106

Similar Datasets

Project description:Peripheral light harvesting (LH) antenna complexes have been studied extensively in the purple nonsulfur bacterium Rhodopseudomonas palustris because it produces different types of LH complexes under high light intensities (LH2 complex) and low light intensities (LH3 and LH4 complex). The ability of R. palustris to alter its peripheral LH complexes in response to changes in light intensity is attributed to the multiple operons that encode the a and b peptides that make up these complexes, whose expression is affected by light intensity, light quality, and oxygen tension. However, low resolution structures, amino acid similarities between the complexes, and a lack of transcriptional analysis made it difficult to determine the LH complexes composition and functions under different light intensities. It was also unclear how much diversity of the R. palustris LH complexes exists in nature.Results: To gain insight into the composition of the LH complexes, their function under high light intensities and low light intensities, and their prevalence in the environment we undertook an integrative genomics approach using 15 closely related R. palustris strains isolated from the environment and 5 R. palustris ecotypes whose genomes have been sequenced. We sequenced the genomes for the 15 closely related strains and using RNA-seq carried out transcriptomic analysis on all 20 strains grown under high light intensity and low light intensity. We were able to determine that even closely related R. palustris strains had differences in their pucBA gene content and expression, even under the same growth conditions. We also found that the LH2 complex could compensate for the lack of an LH4 complex under LL intensities but not under extremely LL intensities. Conclusions: This is the first time an integrative genomics approach has been used to study light harvesting in the environment. The variation observed in LH gene composition and expression in environmental isolates of R. palustris likely reflects how these strains have adapted to specific light conditions in the environment. We have also shown that there is redundancy between some of the LH complexes under certain light intensities, which may partially explain why multiple operons encoding LH complexes have evolved and been maintained in R. palustris. Examing the variation observed in LH gene composition and expression in various environmental isolates