In-depth quantitative proteomic analysis of trophozoites and pseudocysts of Trichomonas vaginalis r

ABSTRACT: Trichomonas vaginalis is a sexually transmitted anaerobic parasite that infects humans causing trichomoniasis, a common and ubiquitous sexually transmitted disease. The life cycle of this parasite presents a trophozoite form without a cystic stage. However, the presence of spherical forms with internalized flagella, non-proliferative, non-motile, viable and reversible, denominated pseudocysts, have been commonly observed in this parasite. To understand the mechanisms involved in the formation of pseudocysts, here we performed a mass spectrometry-based high-throughput quantitative proteomics study using a label-free approach and functional assays by biochemical and flow cytometric methods. We observed that morphological transformation of trophozoite to pseudocysts is coupled to (i) a metabolic shift toward a less glycolytic phenotype, (ii) alterations in the abundance of hydrogenosomal iron-sulfur cluster (ISC) assembly machinery; (iii) increased abundance of regulatory particles of the ubiquitin–proteasome system; (iv) significant alterations in proteins involved in adhesion and cytoskeleton reorganization; (v) arrest in G2/M phase associated to alterations in the abundance of regulatory proteins of the cell cycle. Such data supports that pseudocysts suffer important physiological and structural alterations for survive under unfavorable environmental conditions.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Trichomonas Vaginalis

SUBMITTER: Jacek Wisniewski  

LAB HEAD: Jacek R Wisniewski

PROVIDER: PXD009721 | Pride | 2022-03-01

REPOSITORIES: Pride