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Genital proteins in women at risk of HIV infection

ABSTRACT: In this study, we used a bead-based affinity proteomics method to examine genital proteins in women living in HIV-serodiscordant relationships. Proteins identified using the affinity set-up were validated by a label free tandem mass spectrometry based method in a parallel cohort with concordant results.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cervicovaginal Fluid

SUBMITTER: Frideborg Bradley

LAB HEAD: Adam D Burgener

PROVIDER: PXD009723 | Pride | 2018-12-10

REPOSITORIES: Pride

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Publications

A High-throughput Bead-based Affinity Assay Enables Analysis of Genital Protein Signatures in Women At Risk of HIV Infection.

Månberg Anna A Bradley Frideborg F Qundos Ulrika U Guthrie Brandon L BL Birse Kenzie K Noël-Romas Laura L Lindskog Cecilia C Bosire Rose R Kiarie James J Farquhar Carey C Burgener Adam D AD Nilsson Peter P Broliden Kristina K

Molecular & cellular proteomics : MCP 20181130 3

Women at high risk of HIV infection, including sex workers and those with active genital inflammation, have molecular signatures of immune activation and epithelial barrier remodeling in samples of their genital mucosa. These alterations in the local immunological milieu are likely to impact HIV susceptibility. We here analyze host genital protein signatures in HIV uninfected women, with high frequency of condom use, living in HIV-serodiscordant relationships. Cervicovaginal secretions from wome ...[more]

PMID: 30504243

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